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Cooperative blockade of CK2 and ATM kinases drives apoptosis in VHL-deficient renal carcinoma cells / RNA-seq (BRB-seq) of treated 786-O spheroids

ABSTRACT: Multicellular Tumor Spheroids (MCTS) were pre-formed for 3 days with 786-O cell line to better mimic the tumor microenviroenment. These spheroids were treated with either vehicle (DMSO), drugs alone (KU-60019 10 μM; CX-4945 5 μM) or in combination (KU + CX) for 48h before RNA extraction.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Christophe Battail

PROVIDER: E-MTAB-9977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cooperative Blockade of CK2 and ATM Kinases Drives Apoptosis in VHL-Deficient Renal Carcinoma Cells through ROS Overproduction.

Giacosa Sofia S Pillet Catherine C Séraudie Irinka I Guyon Laurent L Wallez Yann Y Roelants Caroline C Battail Christophe C Evrard Bertrand B Chalmel Frédéric F Barette Caroline C Soleilhac Emmanuelle E Fauvarque Marie-Odile MO Franquet Quentin Q Sarrazin Clément C Peilleron Nicolas N Fiard Gaëlle G Long Jean-Alexandre JA Descotes Jean-Luc JL Cochet Claude C Filhol Odile O

Cancers 20210202 3

Kinase-targeted agents demonstrate antitumor activity in advanced metastatic clear cell renal cell carcinoma (ccRCC), which remains largely incurable. Integration of genomic approaches through small-molecules and genetically based high-throughput screening holds the promise of improved discovery of candidate targets for cancer therapy. The 786-O cell line represents a model for most ccRCC that have a loss of functional pVHL (von Hippel-Lindau). A multiplexed assay was used to study the cellular ...[more]

PMID: 33540838

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