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Antibody array profiling for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions (10X FCS)


ABSTRACT: Antibody arrays with 10X FCS used in Figure 6 of Haab, Dunham, and Brown, Genome Biology 2001 2(2): research0004.1-0004.13

ORGANISM(S): Homo sapiens

SUBMITTER: Sharron Ohgi 

PROVIDER: E-SMDB-107 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions.

Haab B B BB   Dunham M J MJ   Brown P O PO  

Genome biology 20010122 2


<h4>Background</h4>We have developed and tested a method for printing protein microarrays and using these microarrays in a comparative fluorescence assay to measure the abundance of many specific proteins in complex solutions. A robotic device was used to print hundreds of specific antibody or antigen solutions in an array on the surface of derivatized microscope slides. Two complex protein samples, one serving as a standard for comparative quantitation, the other representing an experimental sa  ...[more]

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