Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of yeast treated with NaCl and FK506 vs NaCl

ABSTRACT: Cells were cultured with or without FK506 treatment and exposed to 0.8 M NaCl. Samples were collected from the drug-treated and control cultures at 30 and 45 min after Na+ addition. For the publication, the experiment was repeated twice, and the corresponding time points from each experiment were averaged. The first two arrays and the last two arrays of this set correspond to the two experiments.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Hiroyuki Yoshimoto 

PROVIDER: E-SMDB-13 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiae.

Yoshimoto Hiroyuki H   Saltsman Kirstie K   Gasch Audrey P AP   Li Hong Xia HX   Ogawa Nobuo N   Botstein David D   Brown Patrick O PO   Cyert Martha S MS  

The Journal of biological chemistry 20020610 34

In Saccharomyces cerevisiae, the Ca(2+)/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca(2+) and Na(+), and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a comprehensive analysis of calcineurin/Crz1p-dependent gene expression following addition of Ca(2+) (200 mm) or Na(+) (0.8 m) to yeast. 163 genes exhibited increased expression that was reduced 50%  ...[more]

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