Transcription profiling of wild type vs Pumilio mutant Drosophila
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ABSTRACT: Total RNA was isolated with TRIZOL reagent from wild-type flies (yellow white, yw) and Pumilio mutant flies (pum13; Barker DD. et al. (1992) Genes Dev 6, 2312-26). 12 microgram of total RNA was used for cDNA synthesis using a 1:1 mixture of oligo(dT) and random nonamer primers and in the presence of amino-allyl dUTP.
ORGANISM(S): Drosophila melanogaster
SUBMITTER: Janos Demeter
PROVIDER: E-SMDB-3532 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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