Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human embryonic stem cells during differentiation to more restricted neural progenitors

ABSTRACT: The purpose of this study was to examine global gene expression during the differentiation of human embryonic stem cells to more restricted neural progenitors. We developed an adherent differentiation protocol with completely defined media that produced 2 distinct classes of neuroepithelia based on timing, morphology and expression of known neural markers. The first stage of differentiation is undifferentiated human ES cells (hESCs). The second stage is aggregates of these hESCs after 6 days of separation from supportive mouse embryonic fibroblasts. The third stage is a primitive anterior neuroepithelia that arises after 10 days of differentiation and is marked by a columnar morphology, expression of Pax6 and anterior neural patterning genes. The fourth stage peaks at 17 days when cells form rosettes that resemble the neural tube and express the pan-neural marker Sox1.

ORGANISM(S): Homo sapiens

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-3806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.

Pankratz Matthew T MT   Li Xue-Jun XJ   Lavaute Timothy M TM   Lyons Elizabeth A EA   Chen Xin X   Zhang Su-Chun SC  

Stem cells (Dayton, Ohio) 20070301 6

Understanding neuroectoderm formation and subsequent diversification to functional neural subtypes remains elusive. We show here that human embryonic stem cells (hESCs) differentiate to primitive neuroectoderm after 8-10 days. At this stage, cells uniformly exhibit columnar morphology and express neural markers, including anterior but not posterior homeodomain proteins. The anterior identity of these cells develops regardless of morphogens present during initial neuroectoderm specification. This  ...[more]

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