Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of wild type and mutant axel-4 Arabidopsis seedlings


ABSTRACT: A mutant screen was conducted in Arabidopsis that was based on deregulated expression of auxin-responsive transgenes. Two different tightly regulated (i.e., very low expression in the absence of auxin treatment and very high expression after exogenous auxin treatment) auxin-responsive promoters were used to drive the expression of both a ?-glucuronidase (GUS) reporter gene and a hygromycin phosphotransferase (HPH)?selectable marker gene. This screen yielded several mutants, and five of the mutations (axe1-1 to axe1-5) mapped to the same locus on chromosome 5. A map-based cloning approach was used to locate the axe1 mutations in an Arabidopsis RPD3-like histone deacetylase gene, referred to as HDA6. The axe1 mutant plants displayed increased expression of the GUS and HPH transgenes in the absence of auxin treatment and increased auxin-inducible expression of the transgenes compared with nonmutant control plants. None of a variety of endogenous, natural auxin-inducible genes in the mutant plants were upregulated like the transgenes, however. Results of treatment with the DNA methylation inhibitor 5-aza-2'-deoxycytidine suggest that the axe1 mutations affect transgene silencing; however, histone deacetylase inhibitors had no affect on transgene silencing in mutant or control plants. The specific effect of AtHDA6 mutations on the auxin-responsive transgenes implicates this RPD3-like histone deacetylase as playing a role in transgene silencing. Furthermore, the effect of AtHDA6 on transgene silencing may be independent of its histone deacetylase activity. *

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Janos Demeter 

PROVIDER: E-SMDB-4012 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Identification of Arabidopsis histone deacetylase HDA6 mutants that affect transgene expression.

Murfett J J   Wang X J XJ   Hagen G G   Guilfoyle T J TJ  

The Plant cell 20010501 5


A mutant screen was conducted in Arabidopsis that was based on deregulated expression of auxin-responsive transgenes. Two different tightly regulated (i.e., very low expression in the absence of auxin treatment and very high expression after exogenous auxin treatment) auxin-responsive promoters were used to drive the expression of both a beta-glucuronidase (GUS) reporter gene and a hygromycin phosphotransferase (HPH)-selectable marker gene. This screen yielded several mutants, and five of the mu  ...[more]

Similar Datasets

2007-08-09 | E-GEOD-7587 | biostudies-arrayexpress
2007-08-09 | GSE7587 | GEO
2016-10-01 | GSE73716 | GEO
2024-06-27 | GSE243296 | GEO
2024-06-27 | GSE243255 | GEO
2024-06-27 | GSE252890 | GEO
2017-10-30 | GSE103037 | GEO
2017-10-30 | GSE76406 | GEO
| PRJNA639789 | ENA
2022-03-31 | GSE178565 | GEO