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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of salmon parr brain after exposure to the carbamazepine

ABSTRACT: Concerns have arisen recently over the possible environmental effects of human pharmaceuticals. Although acute toxicities are low, the continuous discharge of pharmaceuticals into the aquatic environment, coupled with the fact that such compounds are selected for use on the basis of a strong pharmacological effect, means that sublethal effects on non-target organisms need to be seriously considered. The juvenile stages of Atlantic salmon are present in many northern European rivers which are also used for the discharge of domestic wastewaters likely to contain pharmaceuticals. One year old salmon parr were exposed to an environmentally relevant concentration (5µg·/ L) of the antidepressant drug carbamazepine for five days and changes of mRNA expression in brain tissues were investigated by means of a custom 17k Atlantic salmon cDNA microarray. The TRAITS 17K Atlantic salmon cDNA microarray was employed. A dual-labelled experimental design was employed for the microarray hybridisations. Each experimental cDNA sample (Cy3 labeled) was competitively hybridised against a common pooled-reference sample (Cy5 labeled). The entire experiment comprised 10 hybridisations - 2 states (CBZ exposed / unexposed) × 1 time-point ( at 5 days) × 5 biological replicates (males only). Hybridisations were undertaken concurrently.

ORGANISM(S): Salmo salar

SUBMITTER: John Taggart

PROVIDER: E-TABM-1065 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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2011-06-01 | E-TABM-1060 | biostudies-arrayexpress

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