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Unknown,Transcriptomics,Genomics,Proteomics

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Response of a resistant pea accesion to Mycosphaerella pinodes

ABSTRACT: Plants of the resistant Pisum sativum subsp. syriacum accession P665 and the susceptible pea cultivar Messire were inoculated with M. pinodes.The experiment was conducted in three replicates. 16, 24 and 48 hours after inoculation RNA was isolated from leaves of infected plants and transcribed into cDNA. For each time point and replicate, Cy-labelled cDNA samples from resistant and susceptible plants were mixed and hybridized to Mt16kOLI1Plus microarray

ORGANISM(S): Pisum sativum

SUBMITTER: Sara Fondevilla

PROVIDER: E-TABM-1084 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology.

Fondevilla Sara S Küster Helge H Krajinski Franziska F Cubero José I JI Rubiales Diego D

BMC genomics 20110113

<h4>Background</h4>Ascochyta blight, caused by Mycosphaerella pinodes is one of the most important pea pathogens. However, little is known about the genes and mechanisms of resistance acting against M. pinodes in pea. Resistance identified so far to this pathogen is incomplete, polygenic and scarce in pea, being most common in Pisum relatives. The identification of the genes underlying resistance would increase our knowledge about M. pinodes-pea interaction and would facilitate the introgression ...[more]

PMID: 21226971

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