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Transcription profiling of barley seedling leaves from Steptoe x Morex doubled-haploid lines and from the parental cultivars

ABSTRACT: We measured mRNA abundance in the seedling leaves of 35 recombinant Steptoe x Morex doubled-haploid lines (no replicates) and in parental genotypes, Steptoe and Morex, 3 replicates each, total 41 chip.

ORGANISM(S): Hordeum vulgare

SUBMITTER: Arnis Druka

PROVIDER: E-TABM-111 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SFP genotyping from affymetrix arrays is robust but largely detects cis-acting expression regulators.

Luo Z W ZW Potokina E E Druka A A Wise R R Waugh R R Kearsey M J MJ

Genetics 20070403 2

The recent development of Affymetrix chips designed from assembled EST sequences has spawned considerable interest in identifying single-feature polymorphisms (SFPs) from transcriptome data. SFPs are valuable genetic markers that potentially offer a physical link to the structural genes themselves. However, most current SFP prediction methodologies were developed for sequenced species although SFPs are particularly valuable for species with complex and unsequenced genomes. To establish the sensi ...[more]

PMID: 17409081

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