DNA methylation in Breast cancer
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ABSTRACT: This experiment was performed with an aim to establish an early diagnostic marker for breast cancer based on the DNA methylation patterns. CpG Island were identified and amplified from the breast cancer related genomic loci. These selected loci were amplified using PCR from different human breast tissue samples. The samples included Breast cancer tissues and macroscopically normal tissues (3-5cm away from tumor) from breast cancer patients, benign breast tissues and healthy breast tissues from cancer free patients. All the genomic loci amplified from one particular tissue sample were pooled, biotin labeled and hybridized to a self designed and synthesised oligonucleotide microarray. The obtained signals were statistically analysed and the genomic loci which could differentiate the different kinds of breast tissues used based on their methylation levels are being reported.
ORGANISM(S): Homo sapiens
DISEASE(S): breast carcinoma
SUBMITTER: Sandeep Kumar Botla
PROVIDER: E-TABM-1171 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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