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Poly(A) tail length profiling of mRNAs from fission yeast

ABSTRACT: To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarrays using total eluate as a reference.

INSTRUMENT(S): Axon GenePix 4000B scanning hardware

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Daniel Lackner

PROVIDER: E-TABM-217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A network of multiple regulatory layers shapes gene expression in fission yeast.

Lackner Daniel H DH Beilharz Traude H TH Marguerat Samuel S Mata Juan J Watt Stephen S Schubert Falk F Preiss Thomas T Bähler Jürg J

Molecular cell 20070401 1

Gene expression is controlled at multiple layers, and cells may integrate different regulatory steps for coherent production of proper protein levels. We applied various microarray-based approaches to determine key gene-expression intermediates in exponentially growing fission yeast, providing genome-wide data for translational profiles, mRNA steady-state levels, polyadenylation profiles, start-codon sequence context, mRNA half-lives, and RNA polymerase II occupancy. We uncovered widespread and ...[more]

PMID: 17434133

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