Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

52 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dm Ladybird over-expression and loss-of-function Expression Profiling

ABSTRACT: In order to identify Ladybird mesodermal and heart target genes, we decided to analyse the transcriptional profiling of embryos with a targeted gain and Loss of function approach. Embryos were collected at stages 11 to 15 of embryogenesis corresponding to the lb expression period. Homozygous ectopically expressing or repressing embryos were collected from a large scale cross of UAS-lbe or UAS-RNAi-lbe flies with 24B-Gal4 or Tin-Gal4 flies, in parallel to stage-matched control. Three independent collections were performed. Total RNA was extracted. Ectopically expression and reference samples were hybridized together on GeneChip Drosophila Genome array2. With this approach, we were able to dicover a repertory of genes involved in different aspects of muscle and heart formation showing a multi-step action of this family of identity genes.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Guillaume Junion

PROVIDER: E-TABM-297 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors.

Junion Guillaume G Bataillé Laetitia L Jagla Teresa T Da Ponte Jean Philippe JP Tapin Romain R Jagla Krzysztof K

Genes & development 20071201 23

Correct diversification of cell types during development ensures the formation of functional organs. The evolutionarily conserved homeobox genes from ladybird/Lbx family were found to act as cell identity genes in a number of embryonic tissues. A prior genetic analysis showed that during Drosophila muscle and heart development ladybird is required for the specification of a subset of muscular and cardiac precursors. To learn how ladybird genes exert their cell identity functions we performed mus ...[more]

PMID: 18056427

Similar Datasets

Transcription profiling time series of Drosophila embryos expressing bagpipe ectopically in the mesoderm

Project description:Expression profiling of D. melanogaster embryos expressing bagpipe ectopically in the mesoderm, measured in a timecourse of embryogenesis. Three one-hour timepoints were assayed (3.5-4.5, 4.5-5.5, 5.5-6.5 hrs after egg laying). Homozygous ectopically expressing embryos were collected using from a large scale cross of UAS-bin and twist-Gal4, 24B-Gal4 flies, in parallel to stage-matched controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Ectopically expression and respective reference samples were hybridized together on INDAC oligo-arrays.

2007-10-11 | E-TABM-248 | biostudies-arrayexpress

Transcription profiling time series of Drosophila embryos expressing biniou ectopically in the mesoderm

Project description:Expression profiling of D. melanogaster embryos expressing biniou ectopically in the mesoderm, measured in a timecourse of embryogenesis. Three one-hour timepoints were assayed (4-5, 5-6, 6-7 hrs after egg laying). Homozygous ectopically expressing embryos were collected using from a large scale cross of UAS-bin and twist-Gal4, 24B-Gal4 flies, in parallel to stage-matched controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Ectopically expression and respective reference samples were hybridized together on INDAC oligo-arrays.

2007-10-11 | E-TABM-251 | biostudies-arrayexpress

Mis-expression of Tramtrack in Drosophila embryos

Project description:We profiled the transcriptome of Drosophila melanogaster embryos in ttk2D50 embryos or after over-expression using btl-GAL4; UAS-ttk, respectively. We further isolated cells that express btl-enh-RFPmoe (Cabernard and Affolter 2005) and FACS sorting, and profiled their transcriptomes in the same genetic backgrounds. A total of 19 samples were analysed with 2-3 replicates each. For each embryos and sorted cells, the genotypes were: ttk2D50 btl-enh-RFPmoe (mutant; embryos: 2, cells: 2), btl-enh-RFPmoe (mutant control; embryos: 2, cells: 3), btl-GAL4; UAS-ttk, btl-enh-RFPmoe (over-expression; embryos: 3, cells: 2), btl-GAL4; btl-enh-RFPmoe (over-expression control; embryos: 3, cells: 2).

2011-11-21 | E-GEOD-30239 | biostudies-arrayexpress

Molecular Organization of Drosophila Neuroendocrine Cells by DIMMED: Global Profiling of Pan-Neuronal DIMMED Expression Effects

Project description:To amass candidate DIMM targets in addition to Phm (Park et al., 2008a), we used genome-wide microarray profiling by over-expressing DIMM throughout the embryonic nervous system. We compared profiles from experimental (elav>dimm) and control (elav-GAL4) embryos at 22-26 hr and 28-32 hr after egg laying (AEL). The design was intended to identify transcripts consistently up-regulated shortly after the induction of DIMM; in so doing, we could circumvent the lethality that ensues in late embryonic, and/ or by early larval stages, due to pan-neuronal DIMM expression. Eight microarrays were used to hybridize cDNAs from experimental dimm::myc-expressing and control flies, sampled at two different embryonic stages. Four microarrays were hybridized with cDNA from the dimm::myc-expressing embryos of genotype w;UAS-dimm2A3/+;elav-GAL4/+; (prefix 'ED'). Another four microarrays were hybridized with cDNAs from the control (maternal) strain: w;;elav-GAL4 (prefix 'E'). Samples ED3 and ED4, E3 and E4 were hybridized with cDNA from stage 14 embryos (24-26hr development at 18C). Samples ED1 and ED2, E1 and E2 were hybridized with cDNA from stage 16 embryos (28-32 hr old embryos at 18C). A pair of GeneChip Drosophila Genome 2.0 arrays (Affymetrix Co., Santa Clara, CA) were tested with each RNA sample (two experimental and two control, for each of two time points). Affymetrix GeneChip Drosophila Genome 2.0 Array

2011-09-05 | E-GEOD-31113 | biostudies-arrayexpress

Tissue-specific ChIP-seq for RNA pol II and H2A.v in Drosophila melanogaster

Project description:ChIP-seq study analysing adult Drosophila melanogaster head, glial, neuronal and fat body, as well as embryonic RNA pol II and H2A.v binding by employing the GAL4-UAS system to generate GFP-fusion proteins and ChIP-seq

2013-08-25 | E-MTAB-1117 | biostudies-arrayexpress

The E3 Ligase CUL3 physically interacts with TANGO10 protein in vivo

Project description:List of protein scores for putative TANGO10 interactors from mass spectrometry analysis of FLAG-tagged TANGO10 using either elav-GAL4 or tim-GAL4 at both ZT10 and ZT 22, as determined using Proteome Discoverer software (ThermoFisher). Fly heads from GAL4 heterozygotes were used as controls. The hits from FLAG-tagged twenty-four (TYF) proteomics were also excluded from the list to increase TANGO10-specificity. Proteins listed are those present in at least one elav-GAL4 sample and at least one tim-GAL4 sample but no negative controls.

2021-11-02 | PXD029326 | Pride

Transcription profiling of Drosophila adults to assess the effect of LARK overexpression in CNS neurons

Project description:The goal of this study is to identify, in the head of adult flies, mRNA species whose expresson level are altered by overexpression of the Drosophila RNA-binding protein LARK in CNS neurons. Experiment Overall Design: RNA samples from adult head of the LARK overexpression flies (elav-gal4; uas-lark/+) and control flies were compared. One total RNA sample was isolated from each genotype, of which three technical replicates (repeating the labeling and hybridization processes) were generated, respectively.

2008-06-14 | E-GEOD-6420 | biostudies-arrayexpress

Gene expression profiles of null Pacman/Xrn1 mutant Drosophila testes

Project description:The null pacman allele pcm[14] causes at lethaility pupariation. Lethality can be rescued by driving expression of wild-type pacman in somatic cells during development using the UAS-GAL4 system. Use of the driver arm-GAL4 allows adult flies to develop, but pacman is not expressed in the germline cells. RNA was prepared from null pacman testes from rescued flies, parental controls (UAS line and GAL4 line) and from a genetic background control for pcm[14] (a line generated by a neutral excision of the P-element used to induce the pcm[14] deletion). Total RNA was prepared from adult Drosophila testes. Sequencing of each genotype was replicated 3 times with a read depth of 12-15 million reads per replicate.

2020-06-01 | E-MTAB-4515 | biostudies-arrayexpress

Comparative analysis of gene expression changes upon overexpression of activated Thickveins in the Drosophila wing at larval vs. pupal stages

Project description:Investigation of whole genome gene expression changes in dissected Drosophila wings at the wandering L3 larval stage and 24h after pupa formation, expressing UAS-activated Thickveins Q-D under control of apterous-gal4 with a tubulin driven temperature sensitive gal80 transgene, compared to the parental strain lacking the UAS-Thickveins transgene A 20 chip study using total RNA recovered from five independently isolated samples of 10 dissected Drosophila wings at the indicated developmental stages expressing UAS-activated thickveins or the non-UAS containing matched control strain.

2012-07-03 | E-GEOD-39056 | biostudies-arrayexpress

Comparative analysis of gene expression changes upon Cabut or dE2F1/dDP overexpression in the Drosophila wing

Project description:Investigation of whole genome gene expression level changes in dissected Drosophila wings at the wandering L3 larval stage, 24h after pupa formation and 36h after pupa formation, expressing either UAS-Cabut or UAS-dE2F1 + UAS-dDP under control of apterous-gal4 with a tubulin driven temperature-sensitive gal80 transgene, compared to the parental strain lacking UAS transgenes. A 36-chip study using total RNA recovered from four independently isolated samples of 10 dissected Drosophila wings at the indicated developmental stages expressing the indicated UAS-transgenes or the non-UAS-containing matched control strain. One sample (2D) did not pass our internal quality control and, therefore, was not included in the analysis. The dE2F1+dDP data included here is further discussed in L. Buttitta, A.J. Katzaroff, B.A. Edgar (2010). A robust cell cycle control mechanism limits E2F-induced proliferation of terminally differentiated cells in vivo. Journal of Cell Biology vol. 189, 981-96 (PMID 20548101). The Cabut data included here is further discussed in A.J. Katzaroff et al. (2011). The Krüppel-like-factor cabut has cell cycle regulatory properties similar to E2F.

2011-07-08 | E-GEOD-30484 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data