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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Dm Ladybird over-expression and loss-of-function Expression Profiling

ABSTRACT: In order to identify Ladybird mesodermal and heart target genes, we decided to analyse the transcriptional profiling of embryos with a targeted gain and Loss of function approach. Embryos were collected at stages 11 to 15 of embryogenesis corresponding to the lb expression period. Homozygous ectopically expressing or repressing embryos were collected from a large scale cross of UAS-lbe or UAS-RNAi-lbe flies with 24B-Gal4 or Tin-Gal4 flies, in parallel to stage-matched control. Three independent collections were performed. Total RNA was extracted. Ectopically expression and reference samples were hybridized together on GeneChip Drosophila Genome array2. With this approach, we were able to dicover a repertory of genes involved in different aspects of muscle and heart formation showing a multi-step action of this family of identity genes.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Guillaume Junion

PROVIDER: E-TABM-297 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors.

Junion Guillaume G Bataillé Laetitia L Jagla Teresa T Da Ponte Jean Philippe JP Tapin Romain R Jagla Krzysztof K

Genes & development 20071201 23

Correct diversification of cell types during development ensures the formation of functional organs. The evolutionarily conserved homeobox genes from ladybird/Lbx family were found to act as cell identity genes in a number of embryonic tissues. A prior genetic analysis showed that during Drosophila muscle and heart development ladybird is required for the specification of a subset of muscular and cardiac precursors. To learn how ladybird genes exert their cell identity functions we performed mus ...[more]

PMID: 18056427

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