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Transcription profiling of mouse prospermatogonia, pachytene oogonia, and gonadal somatic cells from 15 day post-conceptus fetuses and pachytene spermatocytes from adults

ABSTRACT: RNA expression microarray analysis of prospermatogonia in 15 day post-conceptus (dpc) fetuses, a stage when they are undergoing rapid de novo DNA methylation. For comparison, we also analysed 15 dpc pachytene oogonia, 15 dpc female and male gonadal somatic cells, and adult pachytene spermatocytes.

ORGANISM(S): Mus musculus

SUBMITTER: Christophe Lefèvre

PROVIDER: E-TABM-412 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA expression microarray analysis in mouse prospermatogonia: identification of candidate epigenetic modifiers.

Lefèvre Christophe C Mann Jeffrey R JR

Developmental dynamics : an official publication of the American Association of Anatomists 20080401 4

The mammalian totipotent and pluripotent lineage exhibits genome-wide dynamics with respect to DNA methylation content. The first phase of global DNA demethylation and de novo remethylation occurs during preimplantation development and gastrulation, respectively, while the second phase occurs in primordial germ cells and primary oocytes/prospermatogonia, respectively. These dynamics are indicative of a comprehensive epigenetic resetting or reprogramming of the genome in preparation for major dif ...[more]

PMID: 18330932

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