Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of expression diversity in Arabidopsis thaliana


ABSTRACT: The study of natural genetic variation for plant disease resistance responses is a complementary approach to utilizing mutants to elucidate genetic pathways. While some key genes involved in pathways controlling disease resistance, and signaling intermediates such as salicylic acid and jasmonic acid, have been identified through mutational analyses, the use of genetic variation in natural populations permits the identification of change-of-function alleles, which likely act in a quantitative manner. Whole genome microarrays, such as Affymetrix GeneChips, allow for molecular characterization of the disease response at a genomics level and characterization of differences in gene expression due to natural variation. Differences in the level of gene expression, or expression level polymorphisms (ELPs), can be mapped in a segregating population to identify regulatory quantitative trait loci (expression QTLs) affecting host resistance responses. In order to identify an appropriate RIL population to map QTL controlling disease resistance responses, we performed a parental survey of 7 different Arabidopsis accessions. We treated vegetatively grown plants with either salicylic acid or a control solution, and harvested the plants at 3 different time points after chemical treatment. We present Affymetrix GeneChip microarray expression data for 3 biological replications of this parental survey

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Marilyn West 

PROVIDER: E-TABM-51 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Natural variation among Arabidopsis thaliana accessions for transcriptome response to exogenous salicylic acid.

van Leeuwen Hans H   Kliebenstein Daniel J DJ   West Marilyn A L MA   Kim Kyunga K   van Poecke Remco R   Katagiri Fumiaki F   Michelmore Richard W RW   Doerge Rebecca W RW   St Clair Dina A DA  

The Plant cell 20070713 7


Little is known about how gene expression variation within a given species controls phenotypic variation under different treatments or environments. Here, we surveyed the transcriptome response of seven diverse Arabidopsis thaliana accessions in response to two treatments: the presence and absence of exogenously applied salicylic acid (SA), an important signaling molecule in plant defense. A factorial experiment was conducted with three biological replicates per accession with and without applic  ...[more]

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