Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

68 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromatin immunoprecipitation of human centromeric protein A, histone3 K4 and K9 modifications, HP1a and HP1g

ABSTRACT: Chromatin Immunoprecipitation of centromeric protein A, histone3 K4 and K9 modifications, HP1a and HP1g

ORGANISM(S): Homo sapiens

SUBMITTER: Alicia Alonso

PROVIDER: E-TABM-705 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Chromatin immunoprecipitation of centromeric proteins CenpC and CenpH from human cell lines containing a neocentromere in band 13q32

Project description:Chromatin Immunoprecipitation of Centromeric Proteins, CenpA, CenpC or CenpH from human cell lines containing a neocentromere in band 13q32 Processed data files with additional statistics are available for download from <a href="ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/TABM/E-TABM-238/">ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/TABM/E-TABM-238</a>

2008-03-22 | E-TABM-238 | biostudies-arrayexpress

Chromatin immunoprecipitation of centromeric proteins CenpA, CenpC and CenpH from human cell lines which contain either a neocentromere in band 13q21 or no neocentric chromosome

Project description:Chromatin Immunoprecipitation of Centromeric Proteins, CenpA, CenpC or CenpH from cell lines which contain either a neocentromere in band 13q21 or no neocentric chromosomes Processed data files with additional statistics are available for download from <a href="ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/TABM/E-TABM-257/">ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/TABM/E-TABM-257/</a>

2008-03-22 | E-TABM-257 | biostudies-arrayexpress

Chromatin immunoprecipitation of centromeric proteins CenpA and CenpC from human cell lines containing a neocentromere in band 13q21

Project description:Chromatin Immunoprecipitation of Centromeric Proteins, CenpA or CenpC, from human cell lines containing a neocentromere in band 13q21

2006-11-06 | E-TABM-168 | biostudies-arrayexpress

Formation of novel CENP-A domains on tandem repetitive DNA and across chromosome

Project description:Chromatin Immunoprecipitation of Centromeric Protein A. (Note WIG and CHP files are available in the additional.zip archive)

2011-03-01 | E-TABM-1098 | biostudies-arrayexpress

Chromatin immunoprecipitation of centromeric proteins CenpA, CenpC and CenpH from human cell line BBB, which contains a neocentromere in band 13q33.1

Project description:Chromatin Immunoprecipitation of Centromeric Proteins, CenpA, CenpC or CenpH from cell line BBB, which contains a neocentromere in band 13q33.1 Processed data files with additional statistics are available for download from <a href="ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/TABM/E-TABM-245/">ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/TABM/E-TABM-245/</a>

2008-03-22 | E-TABM-245 | biostudies-arrayexpress

The Growth factor independence 1 (GFI1) 36N variant predisposes to Acute Myeloid Leukemia (AML) by inducing epigenetic changes at the Hoxa9 locus

Project description:GFI136N is a coding Single Nucleotide Polymorphism (SNP) in the gene GFI1 that increases the risk for Acute myeloid leukemia (AML) by 60%. It is present in 3-5% of Caucasians and has a prevalence of 12% among AML patients. We generated knockin mice expressing either the human GFI136N variant or the more common GFI136S form and observed that GFI136N, in contrast to GFI136S, lacked the ability to bind to the Gfi1 target gene and leukemia associated transcription factor Hoxa9 in myeloid precursors and failed to initiate the histone modifications that regulate HoxA9 expression. Consistent with this, GFI136N heterozygous AML patients showed increased HOXA9 expression compared to control patients. In the knockin mice, granulo-monocytic pogenitors (GMPs), a bone marrow subset from which AML can arise, show a proliferative expansion in the presence of the GFI136N variant. Finally, the GFI136N variant increased colony formation and proliferation of myeloid precursors induced by the onco-fusion proteins MLL/AF9 or AML1/Eto9a and accelerated the onset of a KRAS-driven myelo-proliferative disease. Our data suggest that GFI136N predisposes to AML by deregulating the expression of Hoxa9, a locus highly relevant for AML, thereby inducing a pre-leukemic state in myeloid precursors that can give rise to AML. 3 samples (2 histone modifications, 1 transcription factor)

2012-12-04 | E-GEOD-31657 | biostudies-arrayexpress

Macro histone variants preserve cell identity by preventing the gain of H3K4me2 during reprogramming

Project description:Transcription factor-induced reprogramming of somatic cells to pluripotency is a very inefficient process, probably due to the existence of important epigenetic barriers that are imposed during differentiation and that contribute to preserve cell identity. In an effort to decipher the molecular nature of these barriers, we followed a genome-wide approach, in which we identified macro histone variants (macroH2A) as highly expressed in human somatic cells but downregulated after reprogramming to pluripotency, as well as strongly induced during differentiation. Knock down of macro histone variants in human keratinocytes increased the efficiency of reprogramming to pluripotency, while overexpression had opposite effects. Genome-wide occupancy profiles show that in human keratinocytes macroH2A.1 preferentially occupies genes that are expressed at low levels and are marked with H3K27me3, including pluripotency-related genes and bivalent developmental regulators, at which its presence prevents the regain of H3K4me2 during reprogramming, over imposing an additional layer of repression that preserves cell identity. Gemone wide occupancy of HA:macroH2A.1 in human keratinocytes

2013-04-03 | E-GEOD-44400 | biostudies-arrayexpress

ChIP-seq of ASY1 and controls on meiotic-stage floral buds of Arabidopsis

Project description:ChIP-seq of ASY1 was carried out on meiotic-stage floral buds of Arabidopsis using an a-ASY1 antibody. The experiment aims to determine the genome-wide profile of ASY1. ASY1 is a component of the chromosome axis and is expressed exclusively during meiosis. Two negative controls were used to test the specificity of the ChIP experiment. First, ChIP-seq using the pre-immune on floral buds was carried out. Second, ChIP-seq using an a-ASY1 antibody was performed on leaf tissue where ASY1 is not expressed.

2020-05-18 | E-MTAB-8705 | biostudies-arrayexpress

BCLAF1 Chip-chip control (siGFP) and BRCA1 depleted (siBRCA1) 293T cells in the absense or presense of Etoposide

Project description:BCLAF1 is a serine-arginine (SR) protein implicated in transcriptional regulation and mRNA splicing. We have recently identified BCLAF1 as part of a novel mRNA splicing complex that is recruited to different genetic promoters by the breast cancer susceptiblity protein, BRCA1 in response to DNA damage. This ChIP-chip study was designed to identify genes/promoters regulated by the BRCA1/BCLAG1 mRNA splicing complex by identifying promoters bound by BCLAF1 in the absense and presense of BRCA1 in control cells and cells treated with etoposide to induce DNA damage. This study includes tripicate BCLAF1 ChIP-chip experiments in untreated and etoposide treated (1uM 16 hours) control cells (siGFP) and cells depleted of BRCA1 (siBRCA1). Chromatin Immunoprecipitaitons were performed in triplicate with BCLAF1 antibodies in control 293T cells transfected with siGFP siRNAs and BRCA1 siRNAs (siBRCA1 to deplete BRCA1). Immunoprecipitated genomic DNA was labelled with Cy3 and Input genomic DNA was labelled with Cy5 and hybridized to NimbleGen human 3x720k RefSeq promoter arrays to identify BCLAF1 boundgenomic DNA regions.

2014-04-25 | E-GEOD-47016 | biostudies-arrayexpress

ChIPseq of DDX3X, DDX54, ERalpha and H3K4me3 in human breast cancer MCF7 cells

Project description:We aimed to investigate the chromatin binding activity of DDX3X and DDX54 RNA helicases in human ER -dependent breast cancer MCF7 cells. We run a parallel chromatin binding profiling of ER ChIP-seq. H3K4me3 profiling was used as a quality control of the ChIP-seq procedure.

2019-11-14 | E-MTAB-6211 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data