Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of wild type and mutants of Sultan V barley plants

ABSTRACT: A large-scale time course expression profiling of wild type (Mla12/Rar1/Rom1) and mutants (mla12-M66, M82 (rar1-1), M100 (rar1-2) and rom1) of barley cultivar Sultan 5 was conducted to understand the molecular mechanisms of delayed powdery mildew resistance. Barley plants were inoculated with powdery mildew pathogen isolate 5874. First leaves of inoculated and non-inoculated plants were harvested at six time points after pathogen inoculation. The experiment was laid out in split-split-plot design with 180 experimental units (3 replications x 2 treatments (inoculated and non-inoculated) x 5 genotypes x 6 time points).

ORGANISM(S): Hordeum vulgare

SUBMITTER: Rico Caldo 

PROVIDER: E-TABM-82 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Stage-specific suppression of basal defense discriminates barley plants containing fast- and delayed-acting Mla powdery mildew resistance alleles.

Caldo Rico A RA   Nettleton Dan D   Peng Jiqing J   Wise Roger P RP  

Molecular plant-microbe interactions : MPMI 20060901 9

Nonspecific recognition of pathogen-derived general elicitors triggers the first line of plant basal defense, which in turn, preconditions the host towards resistance or susceptibility. To elucidate how basal defense responses influence the onset of Mla (mildew resistance locus a)-specified resistance, we performed a meta-analysis of GeneChip mRNA expression for 155 basal defense-related genes of barley (Hordeum vulgare) challenged with Blumeria graminis f. sp. hordei, the causal agent of powder  ...[more]

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