Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of different Arabidopsis genotypes in a time series after infection with Hyaloperonospora parasitica isolates

ABSTRACT: Global transcript profiles of different Arabidopsis thaliana genotypes were examined at 0h, 12h, 24h or 48h post infection with one of three different isolates (races) of the oomycete pathogen Hyaloperonospora parasitica (Hp). The three different isolates of Hp were used for this study, were HpEmoy2, HpHiks1 and HpEmco5 (see Eulgem et al., 2004, Plant Physiol. 135: 1129-1144 for more information). Experimental parameters varied in this study were Arabidopsis genotype, Hp isolate and time after infection.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Thomas Eulgem

PROVIDER: E-TABM-96 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

EDM2 is required for RPP7-dependent disease resistance in Arabidopsis and affects RPP7 transcript levels.

Eulgem Thomas T Tsuchiya Tokuji T Wang Xiao-Jun XJ Beasley Britt B Cuzick Alayne A Tör Mahmut M Zhu Tong T McDowell John M JM Holub Eric E Dangl Jeffery L JL

The Plant journal : for cell and molecular biology 20070123 5

Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7. Although this disease resistance gene encodes a typical nucleotide binding site leucine-rich repeat (NB-LRR) disease resistance protein, its function is independent of the defense hormone salicylic acid and most known genes required for plant immune responses. We identified EDM2 (enhanced downy mildew 2) in a genetic screen for RPP7 suppressors. Mutations of ED ...[more]

PMID: 17253987

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Project description:Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. Due to particular physiological characteristics, no treatments against diseases caused by oomycetes are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phytophthora species and host plants. The present project is focused on the molecular mechanisms that underlie the compatible plant-oomycete interaction and plant disease.The laboratory developed a novel interaction system involving the model plant, Arabidopsis thaliana and Phytophthora parasitica, a soil-borne pathogen infecting a wide host range, thus representing the majority of Phytophthora species. A characteristic feature of the compatible Arabidopsis/Phytophthora parasitica interaction is an extended biotrophic phase, before infection becomes necrotrophic. Because the initial biotrophic phase is extremely short on natural (e.g. solanaceous) hosts, the Arabidopsis system provides the opportunity to analyze, for both interaction partners, the molecular events that determine the initiation of infection and the switch to necrotrophy.The present project aims at analyzing the compatible interaction between A. thaliana roots and Phytophthora parasitica. The Affymetrix A. thaliana full genome chip will be used to characterize modulations of the transcriptome occurring over a period of 24h from the onset of plant root infection to the beginning of necrotrophy. Parallel to this study, a custom designed Phytophthora parasitica biochip will enable analyzing of Phytophthora parasitica gene expression during the same stages. The pathosystem involving A. thaliana and Phytophthora parasitica was described in Attard A, Gourgues M, Callemeyn-Torre N, Keller H. 2010. The New phytologist 187: 449–460. The protocol for recovery of RNA from purified appressoria was described in Kebdani N, Pieuchot L, Deleury E, Panabieres F, Le Berre JY, Gourgues M. 2010. New Phytol 185: 248–257. A series of 14 hybridizations corresponding to two biological replicates each corresponding to RNA extractions of the following biological conditions were used: 1-Vegetative mycelium (recovered from two samples of 4 day-old cultures in liquid V8 medium at 24°C), 2- Motile zoospores (recovered from 8 independent cultures), 3-Appressoria differentiated on onion epidermis (epidermis from 20 onion bulbs inoculated with zoospores collected from 8 independent Petri dishes); appressoria collected 3 hours after inoculation (24 °C), 5- Infection of A. thaliana roots by Phytophthora parasitica zoospores (samples recovered at 2.5, 6, 10.5 and 30 hours post inoculation; 5 inoculated plants for each sample).

Dataset Information

Transcription profiling of different Arabidopsis genotypes in a time series after infection with Hyaloperonospora parasitica isolates

Publications

EDM2 is required for RPP7-dependent disease resistance in Arabidopsis and affects RPP7 transcript levels.

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