Unknown

Dataset Information

0

A conserved pressure-driven mechanism for regulating cytosolic osmolarity.


ABSTRACT: Controlling intracellular osmolarity is essential to all cellular life. Cells that live in hypo-osmotic environments like freshwater must constantly battle water influx to avoid swelling until they burst. Many eukaryotic cells use contractile vacuoles to collect excess water from the cytosol and pump it out of the cell. Although contractile vacuoles are essential to many species, including important pathogens, the mechanisms that control their dynamics remain unclear. To identify basic principles governing contractile vacuole function, we here investigate the molecular mechanisms of two species with distinct vacuolar morphologies from different eukaryotic lineagesâ€"the discoban Naegleria gruberi , and the amoebozoan slime mold Dictyostelium discoideum . Using quantitative cell biology we find that, although these species respond differently to osmotic challenges, they both use actin for osmoregulation, as well as vacuolar-type proton pumps for filling contractile vacuoles. We also use analytical modeling to show that cytoplasmic pressure is sufficient to drive water out of contractile vacuoles in these species, similar to findings from the alveolate Paramecium multimicronucleatum . Because these three lineages diverged well over a billion years ago, we propose that this represents an ancient eukaryotic mechanism of osmoregulation.

SUBMITTER: Velle KB 

PROVIDER: S-EPMC10002747 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

A conserved pressure-driven mechanism for regulating cytosolic osmolarity.

Velle Katrina B KB   Garner Rikki M RM   Beckford Tatihana K TK   Weeda Makaela M   Liu Chunzi C   Kennard Andrew S AS   Edwards Marc M   Fritz-Laylin Lillian K LK  

bioRxiv : the preprint server for biology 20230302


Controlling intracellular osmolarity is essential to all cellular life. Cells that live in hypo-osmotic environments like freshwater must constantly battle water influx to avoid swelling until they burst. Many eukaryotic cells use contractile vacuoles to collect excess water from the cytosol and pump it out of the cell. Although contractile vacuoles are essential to many species, including important pathogens, the mechanisms that control their dynamics remain unclear. To identify basic principle  ...[more]

Similar Datasets

| S-EPMC10529079 | biostudies-literature
| S-EPMC8695382 | biostudies-literature
| S-EPMC11573590 | biostudies-literature
2024-09-12 | GSE276655 | GEO
| S-EPMC6341078 | biostudies-literature
| S-EPMC6426131 | biostudies-literature
| S-EPMC2794784 | biostudies-literature
| S-EPMC8091609 | biostudies-literature
| S-EPMC11550536 | biostudies-literature
| PRJNA1158457 | ENA