Dataset Information

Comprehensive analysis of immune-related genes for classification and immune microenvironment of asthma.

ABSTRACT:

Objectives

To determine the effects of immune-related genes (IRGs) and immune landscape of induced sputum, and develop novel, non-invasive diagnostic molecular therapeutic targets for asthma.

Methods

GSE76262 datasets were used to identify differentially expressed IRGs in asthma. Key IRGs were detected using a protein-protein interaction network. Receiver operating characteristic (ROC) curves were analyzed to investigate the diagnostic value of key IRGs. Gene set enrichment analysis (GSEA) was performed with WebGestalt. Single-sample gene set enrichment analysis and CIBERSORT were used to investigate the immune landscape of induced sputum.

Results

A total of 75 potential IRGs were associated with asthma, most of which were involved in the NF-kappa B signaling pathway. ROC analysis showed AUC values for the hub pathway ranging from 0.676-0.767, with moderate diagnostic value for asthma. We also identified IRGs-related cytokines (TNF-α, IL-1β, IL-8 and IL-6) in 76 asthma and 91 control serum samples to further explore diagnostic efficacy, showing a cumulative AUC of 0.998 for these four related cytokines. Analysis of immune cell infiltration levels showed that follicular helper T cells, activated dendritic cells, activated mast cells and eosinophils were significantly higher and macrophages M0 and macrophages M2 were significantly reduced in sputum from patients with asthma.

Conclusions

IRGs-related cytokines and immune infiltration may contribute to the diagnosis and immune classification of asthma.

SUBMITTER: Feng B

PROVIDER: S-EPMC10006808 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Comprehensive analysis of immune-related genes for classification and immune microenvironment of asthma.

Feng Bin B Zhou Tong T Guo Zhiyi Z Jin Jieyu J Zhang Sheng S Qiu Jun J Cao Jun J Li Jia J Peng Xia X Wang Juan J Xing Yanru Y Ji Renxin R Qiao Longwei L Liang Yuting Y

American journal of translational research 20230215 2

<h4>Objectives</h4>To determine the effects of immune-related genes (IRGs) and immune landscape of induced sputum, and develop novel, non-invasive diagnostic molecular therapeutic targets for asthma.<h4>Methods</h4>GSE76262 datasets were used to identify differentially expressed IRGs in asthma. Key IRGs were detected using a protein-protein interaction network. Receiver operating characteristic (ROC) curves were analyzed to investigate the diagnostic value of key IRGs. Gene set enrichment analys ...[more]

PMID: 36915798

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Dataset Information

Comprehensive analysis of immune-related genes for classification and immune microenvironment of asthma.

Objectives

Methods

Results

Conclusions

Publications

Comprehensive analysis of immune-related genes for classification and immune microenvironment of asthma.

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