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CD115^- monocytic myeloid-derived suppressor cells are precursors of OLFM4^high polymorphonuclear myeloid-derived suppressor cells.

ABSTRACT: Myeloid-derived suppressor cells (MDSCs) consist of monocytic (M-) MDSCs and polymorphonuclear (PMN-) MDSCs that contribute to an immunosuppressive environment in tumor-bearing hosts. However, research on the phenotypic and functional heterogeneity of MDSCs in tumor-bearing hosts and across different disease stage is limited. Here we subdivide M-MDSCs based on CD115 expression and report that CD115^- M-MDSCs are functionally distinct from CD115⁺ M-MDSCs. CD115^- M-MDSCs increased in bone marrow and blood as tumors progressed. Transcriptome analysis revealed that CD115^- M-MDSCs expressed higher levels of neutrophil-related genes. Moreover, isolated CD115^- M-MDSCs had higher potential to be differentiated into PMN-MDSCs compared with CD115⁺ M-MDSCs. Of note, CD115^- M-MDSCs were able to differentiate into both olfactomedin 4 (OLFM4)^hi and OLFM4^lo PMN-MDSCs, whereas CD115⁺ M-MDSCs differentiated into a smaller proportion of OLFM4^lo PMN-MDSCs. In vivo, M-MDSC to PMN-MDSC differentiation occurred most frequently in bone marrow while M-MDSCs preferentially differentiated into tumor-associated macrophages in the tumor mass. Our study reveals the presence of previously unrecognized subtypes of CD115^- M-MDSCs in tumor-bearing hosts and demonstrates their cellular plasticity during tumorigenesis.

SUBMITTER: Zou Y

PROVIDER: S-EPMC10017706 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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CD115- monocytic myeloid-derived suppressor cells are precursors of OLFM4high polymorphonuclear myeloid-derived suppressor cells.

Zou Yunyun Y Kamada Nobuhiko N Seong Seung-Yong SY Seo Sang-Uk SU

Communications biology 20230315 1

Myeloid-derived suppressor cells (MDSCs) consist of monocytic (M-) MDSCs and polymorphonuclear (PMN-) MDSCs that contribute to an immunosuppressive environment in tumor-bearing hosts. However, research on the phenotypic and functional heterogeneity of MDSCs in tumor-bearing hosts and across different disease stage is limited. Here we subdivide M-MDSCs based on CD115 expression and report that CD115- M-MDSCs are functionally distinct from CD115+ M-MDSCs. CD115- M- ...[more]

PMID: 36922564

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Project description:Background Immune cells play a vital role in the pathology of ischemic stroke. Neutrophils and polymorphonuclear myeloid-derived suppressor cells share a similar phenotype and have attracted increasing attention in immune regulation research, yet their dynamics in ischemic stroke remain elusive. Methods and Results Mice were randomly divided into 2 groups and intraperitoneally treated with anti-Ly6G (lymphocyte antigen 6 complex locus G) monoclonal antibody or saline. Distal middle cerebral artery occlusion and transient middle cerebral artery occlusion were applied to induce experimental stroke, and mice mortality was recorded until 28 days after stroke. Green fluorescent nissl staining was used to measure infarct volume. Cylinder and foot fault tests were used to evaluate neurological deficits. Immunofluorescence staining was conducted to confirm Ly6G neutralization and detect activated neutrophils and CD11b+Ly6G+ cells. Fluorescence-activated cell sorting was performed to evaluate polymorphonuclear myeloid-derived suppressor cell accumulation in brains and spleens after stroke. Anti-Ly6G antibody successfully depleted Ly6G expression in mice cortex but did not alter cortical physiological vasculature. Prophylactic anti-Ly6G antibody treatment ameliorated ischemic stroke outcomes in the subacute phase. Moreover, using immunofluorescence staining, we found that anti-Ly6G antibody suppressed activated neutrophil infiltration into parenchyma and decreased neutrophil extracellular trap formation in penumbra after stroke. Additionally, prophylactic anti-Ly6G antibody treatment reduced polymorphonuclear myeloid-derived suppressor cell accumulation in the ischemic hemisphere. Conclusions Our study suggested a protective effect of prophylactic anti-Ly6G antibody administration against ischemic stroke by reducing activated neutrophil infiltration and neutrophil extracellular trap formation in parenchyma and suppressing polymorphonuclear myeloid-derived suppressor cell accumulation in the brain. This study may provide a novel therapeutic approach for ischemic stroke.

Dataset Information

CD115^- monocytic myeloid-derived suppressor cells are precursors of OLFM4^high polymorphonuclear myeloid-derived suppressor cells.

Publications

CD115<sup>-</sup> monocytic myeloid-derived suppressor cells are precursors of OLFM4<sup>high</sup> polymorphonuclear myeloid-derived suppressor cells.

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