Ontology highlight
ABSTRACT:
SUBMITTER: Wang W
PROVIDER: S-EPMC10026036 | biostudies-literature | 2023 Mar
REPOSITORIES: biostudies-literature
Wang Wenshuai W Fedorova Olga O Pyle Anna Marie AM
STAR protocols 20230314 2
Capturing different conformations of receptor proteins that are complexed with ligands by single-particle cryo-EM facilitates our understanding toward the mechanisms of ligand recognition and receptor activation cascades. Here, we present a protocol for capturing RNA-sensing innate immune receptors, such as RIG-I, in multiple conformations by single-particle cryo-EM. We describe steps for protein-ligand sample preparation, data acquisition, and image processing covering focused three-dimensional ...[more]