Dataset Information

Identification of thrombotic biomarkers in orthopedic surgery patients by plasma proteomics.

ABSTRACT:

Background

Due to the poor specificity of D-dimer, more accurate thrombus biomarkers are clinically needed to improve the diagnostic power of VTE.

Methods

The plasma samples were classified into low-risk group (n = 6) and high-risk group (n = 6) according to the Caprini Thrombosis Risk Assessment Scale score. Data-independent acquisition mass spectrometry (DIA-MS) was performed to identify the proteins in the 12 plasma samples. Bioinformatics analysis including volcano plot, heatmap, KEGG pathways and chord diagram analysis were drawn to analyze the significantly differentially expressed proteins (DEPs) between the two groups. Then, another 26 plasma samples were collected to verify the key proteins as potential biomarkers of VTE in orthopedic surgery patients.

Results

A total of 371 proteins were identified by DIA-MS in 12 plasma samples. Volcano plotting showed that there were 30 DEPs. KEGG pathway enrichment analysis revealed that the DEPs were majorly involved in the blood coagulation pathway. The chord diagram analysis demonstrated that proteins SAA1, VWF, FLNA, ACTB, VINC, F13B, F13A and IPSP in the DEPs were significantly related to blood coagulation. VWF and F13B were selected for validation experiments. ELISA test showed that, as compared with those in the low-risk group, the level of VWF in the high-risk sera was significantly increased.

Conclusions

The level of VWF in the high-risk group of thrombosis after orthopedic surgery was significantly higher than that in the low-risk group of preoperative thrombosis, suggesting that VWF may be used as a potential thrombus biomarker in orthopedic surgery patients.

SUBMITTER: Liu CQ

PROVIDER: S-EPMC10028780 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Publications

Identification of thrombotic biomarkers in orthopedic surgery patients by plasma proteomics.

Liu Cui-Qing CQ Gao Yu-Jing YJ Lin Geng-Xiong GX Liang Jun-Ze JZ Li Yan-Fei YF Wang Yi-Chun YC Chen Wen-Yan WY Chen Wei-Ju WJ

Journal of orthopaedic surgery and research 20230321 1

<h4>Background</h4>Due to the poor specificity of D-dimer, more accurate thrombus biomarkers are clinically needed to improve the diagnostic power of VTE.<h4>Methods</h4>The plasma samples were classified into low-risk group (n = 6) and high-risk group (n = 6) according to the Caprini Thrombosis Risk Assessment Scale score. Data-independent acquisition mass spectrometry (DIA-MS) was performed to identify the proteins in the 12 plasma samples. Bioinformatics analysis including volcano plot, heatm ...[more]

PMID: 36944974

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Dataset Information

Identification of thrombotic biomarkers in orthopedic surgery patients by plasma proteomics.

Background

Methods

Results

Conclusions

Publications

Identification of thrombotic biomarkers in orthopedic surgery patients by plasma proteomics.

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