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In vitro and in vivo bioaccessibility, antioxidant activity, and color of red radish anthocyanins as influenced by different drying methods.

ABSTRACT: This study aims to examine the effects of various drying methods, namely vacuum freeze drying (VFD), vacuum drying (VD), hot air drying (HAD), sun drying (SD), and air-impingement jet drying (AIJD), on in vitro and in vivo bioaccessibility of red radish anthocyanins. By color parameters, VFD- and AIJD-dried red radish showed redder color to HAD-, SD-, and VD-dried red radish. SEM images of dried red radish showed multiple holes and loose interior structure. Forty-six anthocyanins were identified in red radish. Original, in vitro and in vivo digestive samples from VFD-dried red radish contained more anthocyanins and were more bioaccessibility than fresh and other dried red radishes. In vitro and in vivo research revealed that dried red radish showed weaker and stronger FRAP and ABTS^·+ scavenging activities than fresh red radish. Colon content of mice had significantly higher FRAP and ABTS^·+ scavenging activities than the stomach, small intestine, and cecum contents.

SUBMITTER: Li W

PROVIDER: S-EPMC10034266 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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In vitro and in vivo bioaccessibility, antioxidant activity, and color of red radish anthocyanins as influenced by different drying methods.

Li Wenfeng W Zhang Yaxi Y Deng Hanlu H Yuan Hong H Fan Xin X Yang Hongyan H Tan Si S

Food chemistry: X 20230310

This study aims to examine the effects of various drying methods, namely vacuum freeze drying (VFD), vacuum drying (VD), hot air drying (HAD), sun drying (SD), and air-impingement jet drying (AIJD), on in vitro and in vivo bioaccessibility of red radish anthocyanins. By color parameters, VFD- and AIJD-dried red radish showed redder color to HAD-, SD-, and VD-dried red radish. SEM images of dried red radish showed multiple holes and loose interior structure. Forty-six anthocyanins w ...[more]

PMID: 36968311

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Project description:IntroductionNutritional content in citrus fruit is enormous. Citrus grandis (L.) Osbeck is underutilised citrus crop that receives little attention due to the lack of knowledge regarding its nutritional value. Citrus waste disposal poses a problem due to economic and environmental factors.MethodsThe metabolites flavonoids, phenols and antioxidant capacity in the dropped fruits of the underutilised citrus species pomelo (Citrus grandis (L.) Osbeck) were examined.Results and discussionHesperidin varied from 1.22 to 2.83% and 1.08 to 1.16% from 10 mm to 14 mm whereas naringin dominates in fruits measuring 10 mm and 12mm with 60.61%, 60.77%, and 47.76%, 45.87% in freeze dried (FD) and hot air oven dried (HAOD) samples. According to the results of the antioxidant assays, the highest concentrations of ABTS azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and DPPH (2, 2-diphenyl-1-picrylhydrazyl radical) were found in freeze dried samples, ranging from 9.679 to 10.416 mmol L-1 Trolox and 14.825 to 16.432 mmol L-1 Trolox, respectively. However, the Ferric Reducing Antioxidant Power (FRAP) assay revealed higher content in samples of both FD and HAOD that were 10mm in size (4.578 mmol L-1 Trolox and 3.730 mmol L-1 Trolox). Total phenol content was measured, and the highest concentrations were found in fruits with a diameter between 10 mm and 18 mm. It ranged from 48.479 to 54.498 mg GAE L-1 in FD samples and from 45.757 to 51.159 mg GAE L-1 in HAOD samples. The smallest fruits, or those that were still in the immature stage, had the highest content. It was found that when the immature dropped fruits were dried by HAOD, the content decreased. At p<0.01 and p<0.05, there was a significant positive correlation between the flavonoids, antioxidants, and total phenols. The results showed that the immature dropped immature fruits of lesser known underutilised citrus sp. Citrus grandis can act as potential source of flavonoids, total phenol concentration, and antioxidant potential. Freeze drying can be recommended to recover the most bioactive substances from physiologically dropped fruits of Citrus grandis for use in the pharmaceutical and nutraceutical sectors. This study will help in reducing the environmental impact caused due to citrus dropped fruits and its responsible management.

Project description:Flower color is an important characteristic that determines the commercial value of ornamental plants. Gentian flowers occur in a limited range of colors because this species is not widely cultivated as a cut flower. Gentiana lutea L. var. aurantiaca (abbr, aurantiaca) is characterized by its orange flowers, but the specific pigments responsible for this coloration are unknown. We therefore investigated the carotenoid and flavonoid composition of petals during flower development in the orange-flowered gentian variety of aurantiaca and the yellow-flowered variety of G. lutea L. var. lutea (abbr, lutea). We observed minor varietal differences in the concentration of carotenoids at the early and final stages, but only aurantiaca petals accumulated pelargonidin glycosides, whereas these compounds were not found in lutea petals. We cloned and sequenced the anthocyanin biosynthetic gene fragments from petals, and analyzed the expression of these genes in the petals of both varieties to determine the molecular mechanisms responsible for the differences in petal color. Comparisons of deduced amino acid sequences encoded by the isolated anthocyanin cDNA fragments indicated that chalcone synthase (CHS), chalcone isomerase (CHI), anthocyanidin synthase 1 (ANS1) and ANS2 are identical in both aurantiaca and lutea varieties whereas minor amino acid differences of the deduced flavonone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR) between both varieties were observed. The aurantiaca petals expressed substantially higher levels of transcripts representing CHS, F3H, DFR, ANS and UDP-glucose:flavonoid-3-O-glucosyltransferase genes, compared to lutea petals. Pelargonidin glycoside synthesis in aurantiaca petals therefore appears to reflect the higher steady-state levels of pelargonidin synthesis transcripts. Moreover, possible changes in the substrate specificity of DFR enzymes may represent additional mechanisms for producing red pelargonidin glycosides in petals of aurantiaca. Our report describing the exclusive accumulation of pelargonidin glycosides in aurantiaca petals may facilitate the modification of gentian flower color by the production of red anthocyanins.

Project description:Two pretreatments (ultrasound sonication [US] and microwave [MW]) and drying techniques (hot air [HA] and freeze-drying [FD]) were used to assess the in vitro bioaccessibility of phenols, carotenoids, and antioxidant activity in three tomato cultivars (Zzx171, Zzx162, and Zzx65). The metabolome-chemometric analysis showed that tomato cultivars dried by FD and HA differed in metabolites. HA samples contained methoxytyrosine and aconitane-type diterpenoids. US-HA enhanced total phenol content bioaccessibility by 64.72%-74.25% in three tomato cultivars after digestion (intestinal phase). US-HA increased the protocatechuic acid levels in all three tomato cultivars in the intestinal phase, but by 55.23% in Zzx65 compared to the undigested samples. Despite all adopted postharvest pretreatments and drying, US-HA retained chlorogenic acid, 4-O-caffeinyliquinic acid, and rutin better, due to microstructure changes in the intestinal phase, and Zzx65 treated with US-HA retained the most. In undigested samples, US-FD improved total carotenoids, lycopene, and β-carotene retention, but the inaccessibility was reduced significantly during in vitro digestion. Cultivar × postharvest treatment affected all phenolic compounds, carotenoids, and antioxidant activities in the intestinal phase. US-HA had improved antioxidant power (FRAP) and 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging activity in the intestinal phase. PRACTICAL APPLICATION: Tomatoes are an important part of the human diet. Their high perishability reduces their nutritional and microbiological quality. The lack of cold storage infrastructure in developing countries contributes to food waste. Convective hot air drying is suitable for recycling fresh market waste products as supplement powders. The ultrasound pretreatment protects bioactive compounds in tomato fruit and is non-thermal. There are several limitations, including equipment cost, energy efficiency, and integration with commercial systems, which limit ultrasound-assisted drying's scalability. The integration of US-HA treatment (ultrasound-assisted hot air drying) could reduce food waste and contribute to the sustainable development goals in agricultural industries.

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In vitro and in vivo bioaccessibility, antioxidant activity, and color of red radish anthocyanins as influenced by different drying methods.

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<i>In vitro</i> and <i>in vivo</i> bioaccessibility, antioxidant activity, and color of red radish anthocyanins as influenced by different drying methods.

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