Ontology highlight
ABSTRACT:
SUBMITTER: Hajdu B
PROVIDER: S-EPMC10036435 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
Hajdu Bálint B Hunyadi-Gulyás Éva É Kato Kohsuke K Kawaguchi Atsushi A Nagata Kyosuke K Gyurcsik Béla B
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 20230223 3
Zinc finger proteins specifically recognize DNA sequences and, therefore, play a crucial role in living organisms. In this study the Zn(II)-, and DNA-binding of 1MEY#, an artificial zinc finger protein consisting of three finger units was characterized by multiple methods. Fluorimetric, circular dichroism and isothermal calorimetric titrations were applied to determine the accurate stability constant of a zinc finger protein. Assuming that all three zinc finger subunits behave identically, the o ...[more]