Dataset Information

Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury via PI3K/Akt/mTOR Pathway.

ABSTRACT: Liver ischemia-reperfusion injury (IRI) is a pathophysiological insult that often occurs during liver surgery. Blackberry leaves are known for their anti-inflammatory and antioxidant activities.

Aims

To achieve site-specific delivery of blackberry leaves extract (BBE) loaded AgNPs to the hepatocyte in IRI and to verify possible molecular mechanisms.

Methods

IRI was induced in male Wister rats. Liver injury, hepatic histology, oxidative stress markers, hepatic expression of apoptosis-related proteins were evaluated. Non-targeted metabolomics for chemical characterization of blackberry leaves extract was performed.

Key findings

Pre-treatment with BBE protected against the deterioration caused by I/R, depicted by a significant improvement of liver functions and structure, as well as reduction of oxidative stress with a concomitant increase in antioxidants. Additionally, BBE promoted phosphorylation of antiapoptotic proteins; PI3K, Akt and mTOR, while apoptotic proteins; Bax, Casp-9 and cleaved Casp-3 expressions were decreased. LC-HRMS-based metabolomics identified a range of metabolites, mainly flavonoids and anthocyanins. Upon comprehensive virtual screening and molecular dynamics simulation, the major annotated anthocyanins, cyanidin and pelargonidin glucosides, were suggested to act as PLA2 inhibitors.

Significance

BBE can ameliorate hepatic IRI augmented by BBE-AgNPs nano-formulation via suppressing, oxidative stress and apoptosis as well as stimulation of PI3K/Akt/mTOR signaling pathway.

SUBMITTER: Fathi AM

PROVIDER: S-EPMC10051224 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Publications

Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury via PI3K/Akt/mTOR Pathway.

Fathi Ahmed M AM Waz Shaimaa S Alaaeldin Eman E Toni Nisreen D M NDM El-Sheikh Azza A K AAK Sayed Ahmed M AM Abdelmohsen Usama Ramadan UR Nazmy Maiiada H MH

Metabolites 20230313 3

Liver ischemia-reperfusion injury (IRI) is a pathophysiological insult that often occurs during liver surgery. Blackberry leaves are known for their anti-inflammatory and antioxidant activities.<h4>Aims</h4>To achieve site-specific delivery of blackberry leaves extract (<i>BBE</i>) loaded AgNPs to the hepatocyte in IRI and to verify possible molecular mechanisms.<h4>Methods</h4>IRI was induced in male Wister rats. Liver injury, hepatic histology, oxidative stress markers, hepatic expression of a ...[more]

PMID: 36984859

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Dataset Information

Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury via PI3K/Akt/mTOR Pathway.

Aims

Methods

Key findings

Significance

Publications

Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury via PI3K/Akt/mTOR Pathway.

Similar Datasets

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