Dataset Information

Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice.

ABSTRACT:

Background

Senescence is characterized by an aggravated inflammatory state that reduces vaccine responsiveness. Dietary supplementation with spray-dried porcine plasma (SDP) exerts anti-inflammatory effects in different mucosal areas. We aimed to determine if the anti-inflammatory properties of SDP improve the efficiency of immunization in senescent animals.

Methods

Experiments were performed in 2-month-old and 6-month-old male SAMP8 mice fed control or SDP (8%) feeds for 4 months. The mice received nasal doses of 2.5 μg of Staphylococcus aureus enterotoxin B (SEB) or vehicle every 15 days (i.e., 3 times). Fifteen days after the last dose, a lethal shock was induced by intraperitoneal administration of SEB and LPS.

Results

Immunization increased anti-SEB IgA in intestinal and bronchoalveolar fluid (p < 0.05). After the lethal shock, all immunized aged mice that were supplemented with SDP survived, in contrast to only 66% of those fed the control feed (p < 0.05). Moreover, after the lethal challenge, aged mice showed higher expression levels of pro-inflammatory cytokines (Il-6, Tnf-α, Ifn-γ, and Il-1β) in jejunal and (Tnf-α, and Il-1β) in lung tissues (p < 0.05), which were reduced by SDP supplementation (p < 0.05). Furthermore, in senescent mice, SDP supplementation augmented Il-4 and Il-10 expression in both tissues (p < 0.05).

Conclusion

SDP reduces the mucosal inflammation associated with aging, improving vaccine protection in senescent mice.

SUBMITTER: Miro L

PROVIDER: S-EPMC10080719 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice.

Miró Lluïsa L Rosell-Cardona Cristina C Amat Concepció C Polo Javier J Moretó Miquel M Pérez-Bosque Anna A

Frontiers in nutrition 20230324

<h4>Background</h4>Senescence is characterized by an aggravated inflammatory state that reduces vaccine responsiveness. Dietary supplementation with spray-dried porcine plasma (SDP) exerts anti-inflammatory effects in different mucosal areas. We aimed to determine if the anti-inflammatory properties of SDP improve the efficiency of immunization in senescent animals.<h4>Methods</h4>Experiments were performed in 2-month-old and 6-month-old male SAMP8 mice fed control or SDP (8%) feeds for 4 month ...[more]

PMID: 37032769

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Dataset Information

Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice.

Background

Methods

Results

Conclusion

Publications

Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice.

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