Dataset Information

3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration.

ABSTRACT: Biomimetic metallic biomaterials prepared for bone scaffolds have drawn more and more attention in recent years. However, the topological design of scaffolds is critical to cater to multi-physical requirements for efficient cell seeding and bone regeneration, yet remains a big scientific challenge owing to the coupling of mechanical and mass-transport properties in conventional scaffolds that lead to poor control towards favorable modulus and permeability combinations. Herein, inspired by the microstructure of natural sea urchin spines, biomimetic scaffolds constructed by pentamode metamaterials (PMs) with hierarchical structural tunability were additively manufactured via selective laser melting. The mechanical and mass-transport properties of scaffolds could be simultaneously tuned by the graded porosity (B/T ratio) and the tapering level (D/d ratio). Compared with traditional metallic biomaterials, our biomimetic PM scaffolds possess graded pore distribution, suitable strength, and significant improvements to cell seeding efficiency, permeability, and impact-tolerant capacity, and they also promote in vivo osteogenesis, indicating promising application for cell proliferation and bone regeneration using a structural innovation.

SUBMITTER: Zhang L

PROVIDER: S-EPMC10087492 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration.

Zhang Lei L Wang Bingjin B Song Bo B Yao Yonggang Y Choi Seung-Kyum SK Yang Cao C Shi Yusheng Y

Bioactive materials 20220716

Biomimetic metallic biomaterials prepared for bone scaffolds have drawn more and more attention in recent years. However, the topological design of scaffolds is critical to cater to multi-physical requirements for efficient cell seeding and bone regeneration, yet remains a big scientific challenge owing to the coupling of mechanical and mass-transport properties in conventional scaffolds that lead to poor control towards favorable modulus and permeability combinations. Herein, inspired by the mi ...[more]

PMID: 37056269

Similar Datasets

Project description:Functionally graded scaffolds (FGSs) designed for bone tissue regeneration exhibit three-dimensional (3D) constructs with spatially varying pores, mirroring the natural bone structure, aiming to offer temporary support and a conducive environment for cells during tissue regeneration in defect sites. While existing research on FGSs has primarily focused on altering pore architecture and tuning biomechanical properties for improved tissue regeneration, limited exploration exists on 3D spatially varying FGSs with multiscale porosity to closely mimic natural bone. In this study, we fabricated and investigated FGSs with macropores varying radially and longitudinally, along with micropores within the struts. Utilizing nonsolvent-induced phase separation integrated with 3D printing, we printed poly(ε-caprolactone) (PCL)/hydroxyapatite (HA) composite scaffolds with both uniform and FG geometries. Two HA content variations (10 and 20 wt %) were employed to assess their impact on scaffold properties. Rheological analysis of polymer suspensions gauged the viscosity and shear stress. Thermogravimetric analysis (thermal gravimetric analysis) determined PCL decomposition and the final HA content in the scaffold. Morphological properties, including porosity, pore size, and pore distribution, were evaluated using microcomputed tomography (micro-CT), while field-emission scanning electron microscopy imaged scaffold surface and cross-sectional morphology. Mechanical tests (compression and tension) assessed the scaffold strength. In vitro assays with MC3T3-E1 preosteoblast cells measured cell viability and alkaline phosphatase enzyme activity in uniform and FGSs with 10% and 20% HA content. Results confirmed that the achieved porosity levels provided sufficient strength and supported effective cell proliferation. Cell culture results demonstrated that uniform scaffolds with 10% HA promoted osteogenesis with slow cell proliferation, whereas FGSs with 20% HA promoted both proliferation and osteogenesis of preosteoblast cells. Overall, the structural, compositional, and biological characterization indicated that both uniform and FGSs provide suitable environments for bone tissue regeneration, with functionally graded scaffold morphology potentially offering a favorable environment for cell response.

Dataset Information

3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration.

Publications

3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets