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First Identification of Fig Virus A and Fig Virus B in Ficus carica in Italy.

ABSTRACT: Extracts of double-stranded RNA from three fig cultivars from an Apulian (Southern Italy) germplasm collection were used for high-throughput sequencing and revealed the presence of two distinct, recently described closteroviruses. Sequences obtained from these Apulian isolates belong to fig virus A and fig virus B and cover 38 and 25% of their RNA genome, respectively. Primer sets designed on selected contigs confirmed the presence of each virus in infected plants. A close phylogenetic relationship, investigated in a fragment of HSP70h protein, occurs among these isolates and the reference genomes. A nucleotide divergence (ranging from 10 to 30% along the different genes) was observed among our isolates and the reference genomes. This is the first finding of these virus species in autochthonous fig accessions in Europe.

SUBMITTER: Amoia SS

PROVIDER: S-EPMC10096765 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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First Identification of Fig Virus A and Fig Virus B in Ficus carica in Italy.

Amoia Serafina Serena SS Chiumenti Michela M Minafra Angelantonio A

Plants (Basel, Switzerland) 20230329 7

Extracts of double-stranded RNA from three fig cultivars from an Apulian (Southern Italy) germplasm collection were used for high-throughput sequencing and revealed the presence of two distinct, recently described closteroviruses. Sequences obtained from these Apulian isolates belong to fig virus A and fig virus B and cover 38 and 25% of their RNA genome, respectively. Primer sets designed on selected contigs confirmed the presence of each virus in infected plants. A close phylogenetic relations ...[more]

PMID: 37050129

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