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A Complete Genome of the Alphaproteobacterial Methanotroph Methylocystis parvus OBBP.

ABSTRACT: A complete genome is presented for Methylocystis parvus OBBP, a Gram-negative aerobic methanotroph of the phylum Alphaproteobacteria. M. parvus OBBP is the genus type strain and of interest in the production of polyhydroxybutyrate and environmental microbiology. The genome consists of two plasmids (248 kbp and 205 kbp) and a chromosome (4.076 Mbp).

SUBMITTER: Claxton Stevens BH

PROVIDER: S-EPMC10112054 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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A Complete Genome of the Alphaproteobacterial Methanotroph Methylocystis parvus OBBP.

Claxton Stevens Benedict H BH Rumah Bashir L BL Stead Christopher E CE Zhang Ying Y

Microbiology resource announcements 20230323 4

A complete genome is presented for Methylocystis parvus OBBP, a Gram-negative aerobic methanotroph of the phylum Alphaproteobacteria. M. parvus OBBP is the genus type strain and of interest in the production of polyhydroxybutyrate and environmental microbiology. The genome consists of two plasmids (248 kbp and 205 kbp) and a chromosome (4.076 Mbp). ...[more]

PMID: 36951565

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Reconstruction of a Genome Scale Metabolic Model of the polyhydroxybutyrate producing methanotroph Methylocystis parvus OBBP.

Project description:BackgroundMethylocystis parvus is a type II methanotroph characterized by its high specific methane degradation rate (compared to other methanotrophs of the same family) and its ability to accumulate up to 50% of its biomass in the form of poly-3-hydroxybutyrate (PHB) under nitrogen limiting conditions. This makes it a very promising cell factory.ResultsThis article reports the first Genome Scale Metabolic Model of M. parvus OBBP. The model is compared to Genome Scale Metabolic Models of the closely related methanotrophs Methylocystis hirsuta and Methylocystis sp. SC2. Using the reconstructed model, it was possible to predict the biomass yield of M. parvus on methane. The prediction was consistent with the observed experimental yield, under the assumption of the so called "redox arm mechanism" for methane oxidation. The co-consumption of stored PHB and methane was also modeled, leading to accurate predictions of biomass yields and oxygen consumption rates and revealing an anaplerotic role of PHB degradation. Finally, the model revealed that anoxic PHB consumption has to be coupled to denitrification, as no fermentation of PHB is allowed by the reconstructed metabolic model.ConclusionsThe "redox arm" mechanism appears to be a general characteristic of type II methanotrophs, versus type I methanotrophs that use the "direct coupling" mechanism. The co-consumption of stored PHB and methane was predicted to play an anaplerotic role replenishing the serine cycle with glyoxylate and the TCA cycle with succinyl-CoA, which allows the withdrawal of metabolic precursors for biosynthesis. The stored PHB can be also used as an energy source under anoxic conditions when coupled to denitrification.

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Poly-3-hydroxybutyrate metabolism in the type II methanotroph Methylocystis parvus OBBP.

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