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Fundamental insights in early-stage inclusion body formation.

ABSTRACT: Early-stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod-shaped protein aggregates, a potential sponge-like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod-shaped inclusion bodies, confirmed their porous morphology, the presence of multiple protein aggregates per cell and modelled inclusion body formation as function of the number of generations.

SUBMITTER: Kopp J

PROVIDER: S-EPMC10128139 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Fundamental insights in early-stage inclusion body formation.

Kopp Julian J Bayer Benjamin B Slouka Christoph C Striedner Gerald G Dürkop Mark M Spadiut Oliver O

Microbial biotechnology 20220713 5

Early-stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod-shaped protein aggregates, a potential sponge-like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod-shaped inclusion bodies, confirmed their porous morphology, the presence of multiple protein aggregates per cell and modelled inclusion body formation as function of the number of generations. ...[more]

PMID: 35830603

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