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Biomimetic lipid-fluorescein probe for cellular bioimaging.

ABSTRACT: Fluorescence probe is one of the most powerful tools for cellular imaging. Here, three phospholipid-mimicking fluorescent probes (FP1-FP3) comprising fluorescein and two lipophilic groups of saturated and/or unsaturated C18 fatty acids were synthesized, and their optical properties were investigated. Like in biological phospholipids, the fluorescein group acts as a hydrophilic polar headgroup and the lipid groups act as hydrophobic non-polar tail groups. Laser confocal microscope images illustrated that FP3, which contains both saturated and unsaturated lipid tails, showed great uptake into the canine adipose-derived mesenchymal stem cells.

SUBMITTER: Moon H

PROVIDER: S-EPMC10160471 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Biomimetic lipid-fluorescein probe for cellular bioimaging.

Moon Hyungkyu H Sultana Tania T Lee JeongIk J Huh Jungrim J Lee Hae Dong HD Choi Myung-Seok MS

Frontiers in chemistry 20230421

Fluorescence probe is one of the most powerful tools for cellular imaging. Here, three phospholipid-mimicking fluorescent probes (FP1-FP3) comprising fluorescein and two lipophilic groups of saturated and/or unsaturated C18 fatty acids were synthesized, and their optical properties were investigated. Like in biological phospholipids, the fluorescein group acts as a hydrophilic polar headgroup and the lipid groups act as hydrophobic non-polar tail groups. Laser confocal microscope images illustra ...[more]

PMID: 37153532

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