Ontology highlight
ABSTRACT:
SUBMITTER: Sosa F
PROVIDER: S-EPMC10160533 | biostudies-literature | 2023 Mar
REPOSITORIES: biostudies-literature
Sosa Froylan F Uh Kyungjun K Drum Jessica N JN Stoecklein Katy S KS Davenport Kimberly M KM Ortega M Sofia MS Lee Kiho K Hansen Peter J PJ
Reproduction & fertility 20230301
The hypothesis that CSF2 plays a role in the preimplantation development of the bovine embryo was tested by evaluating consequences of inactivation of CSF2RA (the functional receptor in the embryo) for development of embryos in utero. CRISPR/Cas9 was used to alter sequences on exon 5 and intron 5 of CSF2RA, Control embryos were injected with Cas9 mRNA only. Embryos > 16 cells at day 5 after insemination were transferred to synchronized recipient females in groups of 7 to 24. Embryos were flushed ...[more]