Unknown

Dataset Information

0

Incomplete dosage balance and dosage compensation in the ZZ/ZW Gila monster (Heloderma suspectum) revealed by de novo genome assembly.


ABSTRACT: Reptiles exhibit a variety of modes of sex determination, including both temperature-dependent and genetic mechanisms. Among those species with genetic sex determination, sex chromosomes of varying heterogamety (XX/XY and ZZ/ZW) have been observed with different degrees of differentiation. Karyotype studies have demonstrated that Gila monsters (Heloderma suspectum) have ZZ/ZW sex determination and this system is likely homologous to the ZZ/ZW system in the Komodo dragon (Varanus komodoensis), but little else is known about their sex chromosomes. Here, we report the assembly and analysis of the Gila monster genome. We generated a de novo draft genome assembly for a male using 10X Genomics technology. We further generated and analyzed short-read whole genome sequencing and whole transcriptome sequencing data for three males and three females. By comparing female and male genomic data, we identified four putative Z-chromosome scaffolds. These putative Z-chromosome scaffolds are homologous to Z-linked scaffolds identified in the Komodo dragon. Further, by analyzing RNAseq data, we observed evidence of incomplete dosage compensation between the Gila monster Z chromosome and autosomes and a lack of balance in Z-linked expression between the sexes. In particular, we observe lower expression of the Z in females (ZW) than males (ZZ) on a global basis, though we find evidence suggesting local gene-by-gene compensation. This pattern has been observed in most other ZZ/ZW systems studied to date and may represent a general pattern for female heterogamety in vertebrates.

SUBMITTER: Webster TH 

PROVIDER: S-EPMC10168389 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Incomplete dosage balance and dosage compensation in the ZZ/ZW Gila monster (<i>Heloderma suspectum</i>) revealed by <i>de novo</i> genome assembly.

Webster Timothy H TH   Vannan Annika A   Pinto Brendan J BJ   Denbrock Grant G   Morales Matheo M   Dolby Greer A GA   Fiddes Ian T IT   DeNardo Dale F DF   Wilson Melissa A MA  

bioRxiv : the preprint server for biology 20230428


Reptiles exhibit a variety of modes of sex determination, including both temperature-dependent and genetic mechanisms. Among those species with genetic sex determination, sex chromosomes of varying heterogamety (XX/XY and ZZ/ZW) have been observed with different degrees of differentiation. Karyotype studies have demonstrated that Gila monsters (<i>Heloderma suspectum</i>) have ZZ/ZW sex determination and this system is likely homologous to the ZZ/ZW system in the Komodo dragon (<i>Varanus komodo  ...[more]

Similar Datasets

| S-EPMC10950046 | biostudies-literature
| S-EPMC4510068 | biostudies-literature
| S-EPMC10170349 | biostudies-literature
| S-EPMC4131918 | biostudies-literature
2015-01-26 | PXD001343 | Pride
| S-EPMC7566651 | biostudies-literature
| S-EPMC3040151 | biostudies-literature
| PRJNA420754 | ENA
| PRJNA189802 | ENA
| PRJNA376377 | ENA