Random transposon mutagenesis of Campylobacter jejuni.
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ABSTRACT:
SUBMITTER: Golden NJ
PROVIDER: S-EPMC101816 | biostudies-literature | 2000 Sep
REPOSITORIES: biostudies-literature
Publications
Random transposon mutagenesis of Campylobacter jejuni.
Golden N J NJ Camilli A A Acheson D W DW
Infection and immunity 20000901 9
Genetic studies of Campylobacter jejuni have been limited due to the lack of a transposon mutagenesis method. Here, we describe a novel technique for random transposon mutagenesis using a mariner-based transposon into C. jejuni strain 480. Insertions were random, as demonstrated by Southern blot analysis and insertional junction sequencing. We have demonstrated, for the first time, random in vivo transposon mutagenesis of C. jejuni. ...[more]
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