Ontology highlight
ABSTRACT:
SUBMITTER: Zeng X
PROVIDER: S-EPMC101877 | biostudies-literature | 2000 Apr
REPOSITORIES: biostudies-literature
Zeng X X Saxild H H HH Switzer R L RL
Journal of bacteriology 20000401 7
Transcription of the Bacillus subtilis dra-nupC-pdp operon is repressed by the DeoR repressor protein. The DeoR repressor with an N-terminal His tag was overproduced with a plasmid under control of a phage T5 promoter in Escherichia coli and was purified to near homogeneity by one affinity chromatography step. Gel filtration experimental results showed that native DeoR has a mass of 280 kDa and appears to exist as an octamer. Binding of DeoR to the operator DNA of the dra-nupC-pdp operon was cha ...[more]