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Complete nucleotide sequence of Tn10.

ABSTRACT: The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal resistance operons, and a hypothetical protein in Bacillus subtilis. The glutamate permease was fully functional when expressed, but Tn10 did not protect Escherichia coli from the toxic effects of various metals.

SUBMITTER: Chalmers R

PROVIDER: S-EPMC102010 | biostudies-literature | 2000 May

REPOSITORIES: biostudies-literature

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Complete nucleotide sequence of Tn10.

Chalmers R R Sewitz S S Lipkow K K Crellin P P

Journal of bacteriology 20000501 10

The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal resistance operons, and a hypothetical protein in Bacillus subtilis. The glutamate permease was fully functional when expressed, but Tn10 did not protect Escherichia coli from the ...[more]

PMID: 10781570

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