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Mechanisms by Which Liposomes Improve Inhaled Drug Delivery for Alveolar Diseases.

ABSTRACT: Diseases of the pulmonary alveolus, such as pulmonary fibrosis, are leading causes of morbidity and mortality, but exceedingly few drugs are developed for them. A major reason for this gap is that after inhalation, drugs are quickly whisked away from alveoli due to their high perfusion. To solve this problem, the mechanisms by which nano-scale drug carriers dramatically improve lung pharmacokinetics using an inhalable liposome formulation containing nintedanib, an antifibrotic for pulmonary fibrosis, are studied. Direct instillation of liposomes in murine lung increases nintedanib's total lung delivery over time by 8000-fold and lung half life by tenfold, compared to oral nintedanib. Counterintuitively, it is shown that pulmonary surfactant neither lyses nor aggregates the liposomes. Instead, each lung compartment (alveolar fluid, alveolar leukocytes, and parenchyma) elutes liposomes over 24 h, likely serving as "drug depots." After deposition in the surfactant layer, liposomes are transferred over 3-6 h to alveolar leukocytes (which take up a surprisingly minor 1-5% of total lung dose instilled) in a nonsaturable fashion. Further, all cell layers of the lung parenchyma take up liposomes. These and other mechanisms elucidated here should guide engineering of future inhaled nanomedicine for alveolar diseases.

SUBMITTER: Ferguson LT

PROVIDER: S-EPMC10231510 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Mechanisms by Which Liposomes Improve Inhaled Drug Delivery for Alveolar Diseases.

Ferguson Laura T LT Ma Xiaonan X Myerson Jacob W JW Wu Jichuan J Glassman Patrick M PM Zamora Marco E ME Hood Elizabeth D ED Zaleski Michael M Shen Mengwen M Essien Eno-Obong EO Shuvaev Vladimir V VV Brenner Jacob S JS

Advanced nanobiomed research 20230127 3

Diseases of the pulmonary alveolus, such as pulmonary fibrosis, are leading causes of morbidity and mortality, but exceedingly few drugs are developed for them. A major reason for this gap is that after inhalation, drugs are quickly whisked away from alveoli due to their high perfusion. To solve this problem, the mechanisms by which nano-scale drug carriers dramatically improve lung pharmacokinetics using an inhalable liposome formulation containing nintedanib, an antifibrotic for pulmonary fibr ...[more]

PMID: 37266328

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Mechanisms by Which Liposomes Improve Inhaled Drug Delivery for Alveolar Diseases.

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Mechanisms by Which Liposomes Improve Inhaled Drug Delivery for Alveolar Diseases.

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