Dataset Information

Glutathione Upregulates the Expression of K_ATP Channels and Vasorelaxation Responses and Inhibits mPTP Opening and Oxidative Stress in the Heart Mitochondria of Old Rats.

ABSTRACT:

Background

In the present work, we investigated the effect of exogenous glutathione in old rats on the expression of ATP-sensitive potassium (K_ATP) channels, the mitochondrial permeability transition pore (mPTP) opening in the heart, and the vasorelaxation responses of isolated aortic rings to activation of K_ATP channels.

Methods

Experiments were performed on adult (6 months) and old (24 months) male Wistar rats, which were divided into three groups: adult, old, and glutathione-treated old rats. Glutathione was injected intraperitoneally at a dose of 52 mg/kg 1 hour before the studies. The mRNA expression of K_ATP channels was determined using reverse transcription and real-time polymerase chain reaction analysis. The effect of glutathione administration on mPTP opening, relaxation responses of isolated aortic rings, and oxidative stress markers was studied.

Results

It was shown that the expression levels of Kir6.1, Kir6.2, and SUR1 subunits of K_ATP channels and levels of reduced glutathione were significantly increased in glutathione-treated old rats (by 8.3, 2.8, 13.1, and 1.5-fold, respectively), whereas the levels of oxidative stress markers (hydrogen peroxide, diene conjugates, malondialdehyde, and rate of superoxide generation) in heart mitochondria and mPTP opening were significantly reduced. Relaxation of aortic rings was significantly increased in response to the actions of K_ATP channel openers flocalin and pinacidil in glutathione-treated animals, which was prevented by glibenclamide.

Conclusions

Thus, the administration of exogenous glutathione to old rats resulted in a significant increase in the expression levels of the Kir6.1, Kir6.2, and SUR1 subunits of K_ATP channels and a decrease in oxidative stress. This was accompanied by inhibition of mPTP opening and enhancement of vasorelaxation responses to activation of K_ATP channels.

SUBMITTER: Strutynskyi R

PROVIDER: S-EPMC10232108 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Glutathione Upregulates the Expression of K<sub>ATP</sub> Channels and Vasorelaxation Responses and Inhibits mPTP Opening and Oxidative Stress in the Heart Mitochondria of Old Rats.

Strutynskyi Ruslan R Strutynska Nataliіa N Mys Lidiia L Goshovska Yulia Y Korkach Yuliia Y Fedichkina Raisa R Okhai Iryna I Strutynskyi Vladyslav V Sagach Vadym V

BioMed research international 20230524

<h4>Background</h4>In the present work, we investigated the effect of exogenous glutathione in old rats on the expression of ATP-sensitive potassium (K<sub>ATP</sub>) channels, the mitochondrial permeability transition pore (mPTP) opening in the heart, and the vasorelaxation responses of isolated aortic rings to activation of K<sub>ATP</sub> channels.<h4>Methods</h4>Experiments were performed on adult (6 months) and old (24 months) male Wistar rats, which were divided into three groups: adult, o ...[more]

PMID: 37265475

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Dataset Information

Glutathione Upregulates the Expression of K_ATP Channels and Vasorelaxation Responses and Inhibits mPTP Opening and Oxidative Stress in the Heart Mitochondria of Old Rats.

Background

Methods

Results

Conclusions

Publications

Glutathione Upregulates the Expression of K<sub>ATP</sub> Channels and Vasorelaxation Responses and Inhibits mPTP Opening and Oxidative Stress in the Heart Mitochondria of Old Rats.

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OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Glutathione Upregulates the Expression of KATP Channels and Vasorelaxation Responses and Inhibits mPTP Opening and Oxidative Stress in the Heart Mitochondria of Old Rats.

Background

Methods

Results

Conclusions

Publications

Glutathione Upregulates the Expression of K<sub>ATP</sub> Channels and Vasorelaxation Responses and Inhibits mPTP Opening and Oxidative Stress in the Heart Mitochondria of Old Rats.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Glutathione Upregulates the Expression of K_ATP Channels and Vasorelaxation Responses and Inhibits mPTP Opening and Oxidative Stress in the Heart Mitochondria of Old Rats.