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Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosis.

ABSTRACT: We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31(+) gene. The prp31-E1 mutant is defective in vegetative cell growth and in meiotic progression. It is synthetically lethal with prp6 and displays a pre-mRNA splicing defect at the restrictive temperature. We cloned the wild-type gene by complementation of the temperature-sensitive mutant phenotype. Prp31p is closely related to human and budding yeast PRP31 homologs and is likely to function as a general splicing factor in both vegetative growth and sexual differentiation.

SUBMITTER: Bishop DT

PROVIDER: S-EPMC102626 | biostudies-literature | 2000 Jun

REPOSITORIES: biostudies-literature

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Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosis.

Bishop D T DT McDonald W H WH Gould K L KL Forsburg S L SL

Nucleic acids research 20000601 11

We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31(+) gene. The prp31-E1 mutant is defective in vegetative cell growth and in meiotic progression. It is synthetically lethal with prp6 and displays a pre-mRNA splicing defect at the restrictive temperature. We cloned the wild-type gene by complementation of the temperature-sensitive mutant phenotype. Prp31p is closel ...[more]

PMID: 10871341

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