Ontology highlight
ABSTRACT:
SUBMITTER: Vu HL
PROVIDER: S-EPMC102801 | biostudies-literature | 2000 Apr
REPOSITORIES: biostudies-literature
Vu H L HL Troubetzkoy S S Nguyen H H HH Russell M W MW Mestecky J J
Nucleic acids research 20000401 7
Accurate quantification of nucleic acids by competitive (RT)-PCR requires a valid internal standard, a reference for data normalization and an adequate mathematical model for data analysis. We report here an effective procedure for the generation of homologous RNA internal standards and a strategy for synthesizing and using a reference target RNA in quantification of absolute amounts of nucleic acids. Further, a new mathematical model describing the general kinetic features of competitive PCR wa ...[more]