Dataset Information

Complete Genome Sequences of Xylella fastidiosa subsp. fastidiosa and X. fastidiosa subsp. multiplex Strains Causing Blueberry Bacterial Leaf Scorch Disease in Georgia, USA.

ABSTRACT: Here, we report the complete genome sequences of Xylella fastidiosa subsp. fastidiosa strain AlmaReb2 and X. fastidiosa subsp. multiplex strain AlmaRebR6, causing blueberry bacterial leaf scorch disease in Georgia, USA. The X. fastidiosa subsp. fastidiosa AlmaRebR2 chromosome is 2,549,422 bp, and the X. fastidiosa subsp. multiplex AlmaReb6 chromosome is 2,530,348 bp.

SUBMITTER: Oliver JE

PROVIDER: S-EPMC10281121 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Complete Genome Sequences of Xylella fastidiosa subsp. fastidiosa and X. fastidiosa subsp. multiplex Strains Causing Blueberry Bacterial Leaf Scorch Disease in Georgia, USA.

Oliver Jonathan E JE Lewis Kippy J KJ Taylor Shae J SJ Chen Jianchi J

Microbiology resource announcements 20230531 6

Here, we report the complete genome sequences of Xylella fastidiosa subsp. fastidiosa strain AlmaReb2 and X. fastidiosa subsp. multiplex strain AlmaRebR6, causing blueberry bacterial leaf scorch disease in Georgia, USA. The X. fastidiosa subsp. fastidiosa AlmaRebR2 chromosome is 2,549,422 bp, and the X. fastidiosa subsp. multiplex AlmaReb6 chromosome is 2,530,348 bp. ...[more]

PMID: 37255489

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Project description:Bacterial leaf scorch (BLS), caused by Xylella fastidiosa (Xf), is a prevalent disease of blueberries in the southeastern United States. Initially, this disease was reported to be caused by X. fastidiosa subsp. multiplex (Xfm). However, a recent survey revealed the presence of another subspecies, X. fastidiosa subsp. fastidiosa (Xff), within naturally infected blueberry plantings in Georgia. Since knowledge regarding the origins of isolates causing Xf outbreaks can impact management recommendations, a routine method for identifying the pathogen at the subspecies level can be beneficial. Several detection strategies are available to identify Xf infection at the subspecies level. However, none of these have been developed for the routine and rapid differentiation of the blueberry-infecting Xf subspecies. Here, we developed two separate straightforward and rapid detection techniques, a cleaved amplified polymorphic sequence (CAPS) marker, and a loop-mediated isothermal amplification (LAMP) assay, targeting the RNA polymerase sigma-70 factor (rpoD) gene sequence of Xfm to discriminate between the two Xf subspecies infecting blueberry. With the CAPS marker, specific detection of Xfm isolates was possible from pure cultures, inoculated greenhouse-grown plant samples, and field infected blueberry samples by restriction digestion of the rpoD gene PCR product (amplified with primers RST31 and RST33) using the BtsI enzyme. The LAMP assay allowed for specific real-time amplification of a 204-bp portion of the XfmrpoD gene from both pure bacterial cultures and infected plant material using the Genie® III system, a result further affirmed by gel electrophoresis and SYBR™ Green I DNA staining for visual observation. These detection strategies have the potential to greatly aid existing diagnostic methods for determining the distribution and prevalence of these Xf subspecies causing bacterial leaf scorch (BLS) in blueberries in the southeastern United States.

Dataset Information

Complete Genome Sequences of Xylella fastidiosa subsp. fastidiosa and X. fastidiosa subsp. multiplex Strains Causing Blueberry Bacterial Leaf Scorch Disease in Georgia, USA.

Publications

Complete Genome Sequences of Xylella fastidiosa subsp. <i>fastidiosa</i> and X. fastidiosa subsp. <i>multiplex</i> Strains Causing Blueberry Bacterial Leaf Scorch Disease in Georgia, USA.

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