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Deep learning enables fast, gentle STED microscopy.

ABSTRACT: STED microscopy is widely used to image subcellular structures with super-resolution. Here, we report that restoring STED images with deep learning can mitigate photobleaching and photodamage by reducing the pixel dwell time by one or two orders of magnitude. Our method allows for efficient and robust restoration of noisy 2D and 3D STED images with multiple targets and facilitates long-term imaging of mitochondrial dynamics.

SUBMITTER: Ebrahimi V

PROVIDER: S-EPMC10300082 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Deep learning enables fast, gentle STED microscopy.

Ebrahimi Vahid V Stephan Till T Kim Jiah J Carravilla Pablo P Eggeling Christian C Jakobs Stefan S Han Kyu Young KY

Communications biology 20230627 1

STED microscopy is widely used to image subcellular structures with super-resolution. Here, we report that restoring STED images with deep learning can mitigate photobleaching and photodamage by reducing the pixel dwell time by one or two orders of magnitude. Our method allows for efficient and robust restoration of noisy 2D and 3D STED images with multiple targets and facilitates long-term imaging of mitochondrial dynamics. ...[more]

PMID: 37369761

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