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Developmental-stage specific single-cell human embryo dissociation.

ABSTRACT: Isolation of individual cells ensures detailed analysis of human embryos and promotes our understanding of molecular mechanisms driving embryo development and cell specification. Here, we present a protocol for the processing of human embryos for single-cell analysis. We describe steps for growing embryos and individualizing cells from the polar and the mural parts of trophectoderm at the blastocyst stage using laser dissection. We then detail embryo dissociation followed by steps to pick, wash, and dispense cells in plates.

SUBMITTER: Lammers J

PROVIDER: S-EPMC10300299 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Developmental-stage specific single-cell human embryo dissociation.

Lammers Jenna J Loubersac Sophie S David Laurent L Freour Thomas T Reignier Arnaud A

STAR protocols 20230617 3

Isolation of individual cells ensures detailed analysis of human embryos and promotes our understanding of molecular mechanisms driving embryo development and cell specification. Here, we present a protocol for the processing of human embryos for single-cell analysis. We describe steps for growing embryos and individualizing cells from the polar and the mural parts of trophectoderm at the blastocyst stage using laser dissection. We then detail embryo dissociation followed by steps to pick, wash, ...[more]

PMID: 37330906

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