Ontology highlight
ABSTRACT:
SUBMITTER: Huang J
PROVIDER: S-EPMC10300391 | biostudies-literature | 2023 Jun
REPOSITORIES: biostudies-literature
Huang Jie J Bao Lijun L Zhu Junyi J Ji Xiong X
STAR protocols 20230615 3
Sequencing chromatin-associated RNA using libraries from the chromatin fraction makes it possible to characterize RNA processing driven by disassociated subunits. Here, we present an experimental strategy and computational pipeline for processing chromatin-associated RNA-seq data to detect and quantify readthrough transcripts. We describe steps for constructing degron mouse embryonic stem cells, detecting readthrough genes, data processing, and data analysis. This protocol can be adapted to vari ...[more]