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High-pressure single-crystal diffraction at the Australian Synchrotron.

ABSTRACT: A new high-pressure single-crystal diffraction setup has been designed and implemented at the Australian Synchrotron for collecting molecular and protein crystal structures. The setup incorporates a modified micro-Merrill-Bassett cell and holder designed specifically to fit onto the horizontal air-bearing goniometer, allowing high-pressure diffraction measurements to be collected with little to no modification of the beamline setup compared with ambient data collections. Compression data for the amino acid, L-threonine, and the protein, hen egg-white lysozyme, were collected, showcasing the capabilities of the setup.

SUBMITTER: Boer SA

PROVIDER: S-EPMC10325021 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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High-pressure single-crystal diffraction at the Australian Synchrotron.

Boer Stephanie A SA Price Jason R JR Riboldi-Tunnicliffe Alan A Williamson Rachel R Rostan Robert R Summers Aston A Turner Gemma F GF Jones Isabelle I Bond Charles S CS Vrielink Alice A Marshall Andrew C AC Hitchings John J Moggach Stephen A SA

Journal of synchrotron radiation 20230615 Pt 4

A new high-pressure single-crystal diffraction setup has been designed and implemented at the Australian Synchrotron for collecting molecular and protein crystal structures. The setup incorporates a modified micro-Merrill-Bassett cell and holder designed specifically to fit onto the horizontal air-bearing goniometer, allowing high-pressure diffraction measurements to be collected with little to no modification of the beamline setup compared with ambient data collections. Compression data for the ...[more]

PMID: 37318368

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