Dataset Information

Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates.

ABSTRACT: Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Trigonellafoenum-graecum); a common ingredient of Indo-Pak cuisines. Briefly, the Fenugreek seeds extract (FgSE) in concentrationsof0.1, 0.5 and 1 mg/ml inhibited the formation of Advanced Glycation End products (AGEs) and fructosamine adducts in Bovine serum albumin (BSA)/fructose model in vitro. The BSA conformational analysis via Circular Dichorism and Congo red assays showed that it preserves secondary structure of BSA in aforementioned model. Although mechanistic studies revealed insignificant lysine blocking ability of Fenugreek by OPA assay, however carbonyl entrapping was found to be 24%, 34% and 42% at 0.1, 0.5 and 1 mg/ml, respectively. In vivo model of High Fructose diet (HFD) induced glycation, FgSE treatment in doses of 10, 25 & 50 mg/kg markedly improved Escape latency (p < 0.01) and preserved cognition in Morris Water Maze. Our data further exhibits significant decrease of CML (Nε-carboxymethyl lysine) levels in serum and hippocampus byFgSE treatment in comparison with HFD group. Therefore, we deduced that FgSE prevents glycation-induced memory decline via entrapping the reactive carbonyl intermediates, formed during production of AGEs. Hence, as a promising functional food it slows down the harmful process of glycation and aging associated morbidities.

SUBMITTER: Anwar L

PROVIDER: S-EPMC10360956 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products <i>via</i> scavenging reactive carbonyl intermediates.

Anwar Laila L Ali Syed Abid SA Khan Sana S Uzairullah Mir Muhammad MM Mustafa Nazish N Ali Urooj Anwer UA Siddiqui Faheema F Bhatti Huma Aslam HA Rehmani Syed Jawad SJ Abbas Ghulam G

Heliyon 20230601 6

Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (<i>Trigonellafoenum-graecum)</i>; a common ingredient of Indo-Pak cuisines. Briefly, the Fenugreek seeds extract (FgSE) in concentrationsof0.1, 0.5 and 1 mg/ml inhibited the formation of Advanced Glycat ...[more]

PMID: 37484294

Similar Datasets

Project description:Annona reticulata L. (Bullock's heart) is a pantropic tree commonly known as custard apple, which is used therapeutically for a variety of maladies. The present research was carried out to evaluate the possible protective effects of Annona reticulata L. (A. reticulata) ethanolic seed extract on an experimentally induced type 2 diabetes rat model. Male Albino Wistar rats were randomly divided into five groups with six animals in each group viz., control rats in group I, diabetic rats in group II, diabetic rats with 50 and 100 mg/kg/bw of ethanolic seed extract of A. reticulata in groups III and IV, respectively, and diabetic rats with metformin in group V. Treatment was given for 42 consecutive days through oral route by oro-gastric gavage. Administration of A. reticulata seed extract to diabetes rats significantly restored the alterations in the levels of body weight, food and water intake, fasting blood glucose (FBG), insulin levels, insulin sensitivity, HbA1c, HOMA-IR, islet area and insulin positive cells. Furthermore, A. reticulata significantly decreased the levels of triglycerides, cholesterol, LDL, and significantly increased the HDL in diabetic rats. A. reticulata effectively ameliorated the enzymatic (ALT, AST, ALP, GGT) and modification of histopathological changes in diabetic rats. The serum levels of the BUN, creatinine levels, uric acid, urine volume, and urinary protein were significantly declined with a significant elevation in CCr in diabetic rats treated with A. reticulata. MDA and NO levels were significantly reduced with an enhancement in SOD, CAT, and GPx antioxidant enzyme activities in the kidney, liver, and pancreas of diabetic rats treated with A. reticulata. Diabetic rats treated with A. reticulata have shown up-regulation in mRNA expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), NAD(P)H:quinone oxidoreductase 1 (NQO1), Heme oxygenase-1 (HO-1) and protein expression level of Nrf2 with diminution in Keap1 mRNA expression level in pancreas, kidney, and liver. From the outcome of the current results, it can be inferred that seed extract of A. reticulata exhibits a protective effect in diabetic rats through its anti-diabetic, anti-hyperlipidemic, antioxidant and anti-inflammatory effects and could be considered as a promising treatment therapy in the treatment of diabetes mellitus.

Project description:Abstract Objectives Excessive consumption of advanced glycation end products (AGEs) with western-style diet in vogue is increasingly seen as a potential cause of aging and metabolic diseases. Several berries contain appreciable quantities of bioactive phytonutrients, including phenolic compounds, which are potentially good candidates as AGE inhibitors. This parallel double-blind intervention study investigated whether the Vaccinium Myrtillus extract supplement is able to reduce the AGEs levels in humans. (clinicaltrials.gov NCT03316612) Methods Seventy-four healthy subjects were randomly assigned to an intervention group (4 tablets per day, containing 600 mg Vaccinium Myrtillus extract, n = 39) versus placebo group (tablets with the same appearance but without the berry extract, n = 35). We evaluated dietary intake, sleep, exercise, mental state, and anthropometric characteristics over the 3 months of intervention. Plasma soluble receptor for AGE (sRAGE) levels were determined by ELISA (R&D). Plasma protein-bound AGEs, including Nε-(carboxymethyl)lysine (CML) and Nε-(carboxyethyl)lysine (CEL), were determined by ultra-high performance liquid chromatography-tandem mass spectrometry. Results Dietary intake, sleep, exercise, mental state, anthropometric characteristics, and plasma sRAGE levels were not changed during the intervention in two groups. CML levels [1137.5 (918.5–1493.0) vs. 840.9 (757.4–953.8) μg/L, P < 0.001] decreased significantly in the intervention group compared with the placebo group. However, we did not observe a significant change of CEL levels [201.9 (168.5–236.3) vs.176.8 (147.1–212.6) μg/L, P = 0.067] in the intervention group. Conclusions Our results suggest that plasma protein-bound AGEs can be reduced partly by Vaccinium Myrtillus extract supplement. As widely available, safe and nutritious foods, berries with rich phenolic metabolites (particularly anthocyanins) represent a promising dietary intervention worthy of further investigation. Funding Sources Nutrition Scientific Research Foundation of BY-HEALTH. Supporting Tables, Images and/or Graphs

Dataset Information

Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates.

Publications

Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products <i>via</i> scavenging reactive carbonyl intermediates.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets