Analysis of protein synthesis rates after initiation of chromosome replication in Escherichia coli.

ABSTRACT: The aim of this study was to investigate whether the synthesis rates of some proteins change after the initiation of replication in Escherichia coli. An intR1 strain, in which chromosome replication is under the control of an R1 replicon integrated into an inactivated oriC, was used to synchronize chromosome replication, and the rates of protein synthesis were analyzed by two-dimensional polyacrylamide gel electrophoresis of pulse-labeled proteins. Computerized image analysis was used to search for proteins whose expression levels changed at least threefold after initiation of a single round of chromosome replication, which revealed 7 out of about 1,000 detected proteins. The various synthesis rates of three of these proteins turned out to be caused by unbalanced growth and the synthesis o

SUBMITTER: Bechtloff D 

PROVIDER: S-EPMC103762 | biostudies-literature | 1999 Oct

REPOSITORIES: biostudies-literature