Dataset Information

Mapping QTL conferring flag leaf senescence in durum wheat cultivars.

ABSTRACT: Flag leaf senescence is a critical factor affecting the yield and quality of wheat. The aim of this study was to identify QTLs associated with flag leaf senescence in an F₁₀ recombinant inbred line population derived from durum wheats UC1113 and Kofa. Bulked segregant analysis using the wheat 660K SNP array identified 3225 SNPs between extreme-phenotype bulks, and the differential SNPs were mainly clustered on chromosomes 1A, 1B, 3B, 5A, 5B, and 7A. BSR-Seq indicated that the significant SNPs were mainly located in two intervals of 354.0-389.0 Mb and 8.0-15.0 Mb on 1B and 3B, respectively. Based on the distribution of significant SNPs on chromosomes 1B and 3B, a total of 109 insertion/deletion (InDel) markers were developed, and 8 of them were finally used to map QTL in UC1113/Kofa population for flag leaf senescence. Inclusive composite interval mapping identified two major QTL in marker intervals Mar2005-Mar2116 and Mar207-Mar289, explaining 14.2-15.4% and 31.4-68.6% of the phenotypic variances across environments, respectively. Using BSR-Seq, gene expression and sequence analysis, the TraesCS1B02G211600 and TraesCS3B02G023000 were identified as candidate senescence-associated genes. This study has potential to be used in cloning key genes for flag leaf senescence and provides available molecular markers for genotyping and marker-assisted selection breeding.

Supplementary information

The online version contains supplementary material available at 10.1007/s11032-023-01410-3.

SUBMITTER: Ren Y

PROVIDER: S-EPMC10409934 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Mapping QTL conferring flag leaf senescence in durum wheat cultivars.

Ren Yan Y Sun Xiaonan X Nie Jingyun J Guo Peng P Wu Xiaohui X Zhang Yixiao Y Gao Mengjuan M Niaz Mohsin M Yang Xia X Sun Congwei C Zhang Ning N Chen Feng F

Molecular breeding : new strategies in plant improvement 20230808 8

Flag leaf senescence is a critical factor affecting the yield and quality of wheat. The aim of this study was to identify QTLs associated with flag leaf senescence in an F<sub>10</sub> recombinant inbred line population derived from durum wheats UC1113 and Kofa. Bulked segregant analysis using the wheat 660K SNP array identified 3225 SNPs between extreme-phenotype bulks, and the differential SNPs were mainly clustered on chromosomes 1A, 1B, 3B, 5A, 5B, and 7A. BSR-Seq indicated that the signific ...[more]

PMID: 37564974

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Dataset Information

Mapping QTL conferring flag leaf senescence in durum wheat cultivars.

Supplementary information

Publications

Mapping QTL conferring flag leaf senescence in durum wheat cultivars.

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