Ontology highlight
ABSTRACT:
SUBMITTER: Willett JLE
PROVIDER: S-EPMC10434011 | biostudies-literature | 2023 Aug
REPOSITORIES: biostudies-literature
Willett Julia L E JLE Barnes Aaron M T AMT Brunson Debra N DN Lecomte Alexandre A Robertson Ethan B EB Dunny Gary M GM
Microbiology spectrum 20230711 4
Biological collections, including arrayed libraries of single transposon (Tn) or deletion mutants, greatly accelerate the pace of bacterial genetic research. Despite the importance of these resources, few protocols exist for the replication and distribution of these materials. Here, we describe a protocol for creating multiple replicates of an arrayed bacterial Tn library consisting of approximately 6,800 mutants in 96-well plates (73 plates). Our protocol provides multiple checkpoints to guard ...[more]