Dataset Information

Identification and genotyping of Acanthamoeba spp. in the water resources of western Iran.

ABSTRACT:

Background

Acanthamoeba spp. is opportunistic amoeba that resides in water, soil, and air. Some pathogenic genotypes of the genus of Acanthamoeba can cause granulomatous amoebic encephalitis (GAE) in people with a defective immune system. The parasite can also cause Acanthamoeba keratitis (AK) among contact lens users. This study was conducted to isolate and identify the Acanthamoeba genotypes in water resources in Lorestan province, western Iran.

Methods

Collected 72 water samples from surface and groundwater (springs and aqueducts) in Lorestan province. Samples were filtered and cultured in non-nutrient 1.5% agar medium covered with Escherichia coli (E. coli) at 25 °C. DNA extraction was done and the PCR reaction was performed to detect the Acanthamoeba spp. The positive PCR products were sequenced to determine the genotypes of Acanthamoeba.

Results

Out of 72 examined water samples, 23.61% were positive for Acanthamoeba sp. by PCR. From PCR-positive samples, 8 (47.05%) samples were T4 genotypes and others were other Acanthamoeba genotypes (T1-T23). Therefore, approximately half of the genotypes belong to the pathogenic T4 genotype.

Conclusions

The water examined samples in western provinces of Iran have the potential risk factor for public health. Therefore, the efforts of healthcare providers are needed to identify, train, and prevention from human infections.

SUBMITTER: Karimi A

PROVIDER: S-EPMC10450349 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Publications

Identification and genotyping of <i>Acanthamoeba</i> spp. in the water resources of western Iran.

Karimi Azadeh A Kheirandish Farnaz F Javadi Mamaghani Amirreza A Taghipour Niloofar N Mousavi Syedeh Fatemeh SF Aghajani Ali A Zebardast Nozhat N Faraji Majid M Fallahi Shirzad S

Parasite epidemiology and control 20230525

<h4>Background</h4><i>Acanthamoeba</i> spp. is opportunistic amoeba that resides in water, soil, and air. Some pathogenic genotypes of the genus of <i>Acanthamoeba</i> can cause granulomatous amoebic encephalitis (GAE) in people with a defective immune system. The parasite can also cause <i>Acanthamoeba</i> keratitis (AK) among contact lens users. This study was conducted to isolate and identify the <i>Acanthamoeba</i> genotypes in water resources in Lorestan province, western Iran.<h4>Methods</ ...[more]

PMID: 37638113

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